Sequences from confirmation amplicon


Confirmation sequences for line 005G04 and hit At1g03260 are shown.
Line ID 005G04
Locus At1g03260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:796846
Primers used for PCR 01by - 8474, show primer details
   
Sequence Name 18-K108223-0022-005-G04C-01by
GenBank Accession LN494337 [GenBank]
Sequencing primer gene specific primer, 01by: 5'-CAAGCTCATCTACAATCATTTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K108223-0022-005-G04C-01by
ATGAGCGAAACTTACCCACGAAATACTGACACCTCATGCCATCCATGTGTAATATCAGAA
AGATCTTTCAGAGTAGTTCCAACATATACTAGCGCAAATGTAATGGGCTGCTACATCAAA
ACAATTCAGAAGATTAGAATTACTATTATGTATAATGTTAGCAATCAGCACTTTAACATG
CATGCTAACTTCTTCCACCATTATTAAAGTATAAATGGCCAATATACAAGCGGATTTTAA
AGAAATGTCAGTACCATCATTCCCAACCAAGTGGCCAGCATGTATTCCCCTAAGCGAACA
GGAGTTACAGATAGGAGGTCATTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 02-K108222-0022-005-G04C-8409
GenBank Accession LN487065 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K108222-0022-005-G04C-8409
TTGAATATATCCTGAATGACCTCCTATCTGTAACTCCTGTTCGCTTAGGGGAATACATGC
TGGCCACTTGGTTGGGAATGATGGTACTGACATTTCTTTAAAATCCGCTTGTATATTGGC
CATTTATACTTTAATAATGGTGGAAGAAGTTAGCATGCATGTTAAAGTGCTGATTGCTAA
CATTATACATAATAGTAATTCTAATCTTCTGAATTGTTTTGATGTAGCAGCCCATTACAT
TTGCGCTAGTATATGTTGGAACTACTCTGAAAGATCTTTCTGATATTACACATGGATGGC
ATGAGGTGTCAGTATTTCGTTGGGTAAGTTTTCGCTCATTTACTTCCACTTGACTAGTAG
ATTGGAAATGATTGTAGATGAGCTTG

Last Updated on 06.12.2018 16:11