Sequences from confirmation amplicon


Confirmation sequences for line 013E09 and hit At1g05570 are shown.
Line ID 013E09
Locus At1g05570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1650909
Primers used for PCR VL36 - 8474, show primer details
   
Sequence Name 09-K037321-022-013-E09C-8409
GenBank Accession LN490089 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K037321-022-013-E09C-8409
TAGTTAGCTTGCTATTTCCTCATGGCAGGTATCCGTCTATTCGTGTCGCCTATATCGATG
AGGTAGAGCAAACACATAAAGAAAGTTATAAGGGGACAGAGGAGAAAATTTATTACTCAG
CGCTTGTAAAAGCTGCTCCACAGACCAAACCAATGGATTCTTCTGAATCTGTTCAAACAC
TAGATCAGGTATAAGTTGAGAAAACTAAGTAAAATACTTAACATTTTGGTGCTCCTTTTT
TTCCCAAAATACGAGGATGAGTTTAATCCTATGCAGTGAGGTCAGAGGATATAGATGCTG
CTGTTCAAGAGAGACTGAGTTATATTTATTTTCTGTCAGCTCATTTATCGGATTAAGCTT
CCGGGACCAGCTATACTAGGAGAGGGAAAGCCGGAAAATCAGAACCATGCTATCATATTT
ACCCGTGGAGAAGGGTTGCAGACGATAGACATGAATCAGGTGCGATGCGTAGTTTCTTTT
TGTCT
   
Sequence Name 01-K037321-022-013-E09C-VL36
GenBank Accession LN486485 [GenBank]
Sequencing primer gene specific primer, VL36: 5'-TAGACAAAAGAAAACTACGCATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K037321-022-013-E09C-VL36
CGTCTGCACCCTTCTCCACGGGTAAATATGATAGCATGGTTCTGATTTTCCGGCTTTCCC
TCTCCTAGTATAGCTGGTCCCGGAAGCTTAATCCGATAAATGAGCTGACAGAAAATAAAT
ATAACTCAGTCTCTCTTGAACAGCAGCATCTATATCCTCTGACCTCACTGCATAGGATTA
AACTCATCCTCGTATTTTGGGAAAAAAAGGAGCACCAAAATGTTAAGTATTTTACTTAGT
TTTCTCAACTTATACCTGATCTAGTGTTTGAACAGATTCAGAAGAATCCATTGGTTTGGT
CTGTGGAGCAGCTTTTACAAGCGCTGAGTAATAAATTTTCTCCTCTGTCCCCTTATAACT
TTCTTTATGTGTTTGCTCTACCTCATCGATATAGGCGACACGAATAGACGGATACCTGCC
ATGAGGAAATAGCAAGCTTAACTAATGAGGCTAAGAACTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC
GACGTGTCTACATTCACTCCAAAATGG
Primers used for PCR PD19 - 8474, show primer details
   
Sequence Name 10-K037321-022-013-E09C-oril
GenBank Accession LN490564 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K037321-022-013-E09C-oril
GCTTGCTATTTCCTCATGGCAGGTATCCGTCTATTCGTGTCGCCTATATCGATGAGGTAG
AGCAAACACATAAAGAAAGTTATAAGGGGACAGAGGAGAAAATTTATTACTCAGCGCTTG
TAAAAGCTGCTCCACAGACCAAACCAATGGATTCTTCTGAATCTGTTCAAACACTAGATC
AGGTATAAGTTGAGAAAACTAAGTAAAATACTTAACATTTTGGTGCTCCTTTTTTTCCCA
AAATACGAGGATGAGTTTAATCCTATGCAGTGAGGTCAGAGGATATAGATGCTGCTGTTC
AAGAGAGACTGAGTTATATTTATTTTCTGTCAGCTCATTTATCGGATTAAGCTTCCGGGA
CCAGCTATACTAGGAGAGGGAAAGCCGGAAAATCAGAACCATGCTATCATATTTACCCGT
GGAGAAGGGTTGCAGACGATAGACATGAATCAGGTGCGATGCGTAGTTTTCTTTTGTCTA
ACATGTGTCGCTAATGAATCACATATAATATGCTTAAAATTGTTCAAAGAATAATCACCG
CAGGAGACTCTTAACCTCTAAATATTTGACCTTAGGACAACTACATGGAGGAGCCTTTTC
AAA
   
Sequence Name 02-K037321-022-013-E09C-PD19
GenBank Accession LN486941 [GenBank]
Sequencing primer gene specific primer, PD19: 5'-TTTGAAAGCTTCCTCCATGTAGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K037321-022-013-E09C-PD19
GAGGTTAGAGTCTCCTGCGGTGATTATTCTTTGAACAATTTTAAGCATATTATATGTGAT
TCATTAGCGACACATGTTAGACAAAAGAAAACTACGCATCGCACCTGATTCATGTCTATC
GTCTGCAACCCTTCTCCACGGGTAAATATGATAGCATGGTTCTGATTTTCCGGCTTTCCC
TCTCCTAGTATAGCTGGTCCCGGAAGCTTAATCCGATAAATGAGCTGACAGAAAATAAAT
ATAACTCAGTCTCTCTTGAACAGCAGCATCTATATCCTCTGACCTCACTGCATAGGATTA
AACTCATCCTCGTATTTTGGGAAAAAAAGGAGCACCAAAATGTTAAGTATTTTACTTAGT
TTTCTCAACTTATACCTGATCTAGTGTTTGAACAGATTCAGAAGAATCCATTGGTTTGGT
CTGTGGAGCAGCTTTTACAAGCGCTGAGTAATAAATTTTCTCCTCTGTCCCCTTATAACT
TTCTTTATGTGTTTGCTCTACCTCATCGATATAGGCGACACGAATAGACGGATACCTGCC
ATGAGGAAATAGCAAGCTTAACTAATGAGGCTAAGAACTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTC
GACGTGTCTAC

Last Updated on 06.12.2018 16:11