Sequences from confirmation amplicon


Confirmation sequences for line 014B05 and hit At3g28650 are shown.
Line ID 014B05
Locus At3g28650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10737698
Primers used for PCR 32kb - 8474, show primer details
   
Sequence Name 53-K113379-0022-014-B05C-32kb
GenBank Accession LN508213 [GenBank]
Sequencing primer gene specific primer, 32kb: 5'-ATTGACGAAAAAGAAGTCGTCCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K113379-0022-014-B05C-32kb
TTTGGATTTCGTTGTGCCACTCTACCATATACAGTAAGGCTTCCCAGGTACGATGATCAT
CCACTCACTCTTTGTTATGGTGAAAAGGCAAGCGGAAAATATTGGTGTGATATTTGCGAA
AGAGAAACAAATTCAAAGACTTGGTTCTACACTTGTAAAGGTTGCGGGGTCACTTTGCAT
GTCTTTTGTGTACTTGGGGATATTCGATATGCTAAACCAGGAGGAAGCATCAACAACAAT
GTTGAATTGGTGCCTAACAATAGATCTTCAAGACCACTTTGCCACAATTGTCATTGTCAT
TGCCCAGGTCCCTTCATTCTTAAGGATTTTGTCGAAGAATGTTTTTGTTCTTACTATTGT
TTCAGCCGTTGCAAATCCTGGATGTCCATCGTGAAGAAGGAAAAGAGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATTA
   
Sequence Name 15-K113377-0022-014-B05C-8409
GenBank Accession LN493051 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K113377-0022-014-B05C-8409
ATTTCTCTTTTCCTTCTTCACGATGGACATCCAGGATTTGCAACGGCTGAAACAATAGTA
AGAACAAAAACATTCTTCGACAAAATCCTTAAGAATGAAGGGACCTGGGCAATGACAATG
ACAATTGTGGCAAAGTGGTCTTGAAGATCTATTGTTAGGCACCAATTCAACATTGTTGTT
GATGCTTCCTCCTGGTTTAGCATATCGAATATCCCCAAGTACACAAAAGACATGCAAAGT
GACCCCGCAACCTTTACAAGTGTAGAACCAAGTCTTTGAATTTGTTTCTCTTTCGCAAAT
ATCACACCAATATTTTCCGCTTGCCTTTTCACCATAACAAAGAGTGAGTGGATGATCATC
GTACCTGGGAAGCCTTACTGTATATGGTAGAGTGGCACAACGAAAATCCAAAAAGTAGTT
GCATTTTAAACAACCTAGGACGACTTCTTTATCGTCAA

Last Updated on 06.12.2018 16:11