Sequences from confirmation amplicon


Confirmation sequences for line 022D04 and hit At5g37180 are shown.
Line ID 022D04
Locus At5g37180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14719897
Primers used for PCR MS12 - 8474, show primer details
   
Sequence Name 03-K016382-022-022-D04C-8409
GenBank Accession LN487268 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K016382-022-022-D04C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCCCGGGAAAGCT
TGAGATGAGTTTGATTTTGGCAGATGTTTTCCTATCGGAGCTGCCTAAGGACACACCTTT
CCAAGCCTTTGAACTCAGGTTAAACCTTTAATAATTTACATTGCTATATCGTATAGTGAT
ATTACCACAATATGTTTTGTTGCAGTAACTCAGCAAGTCGTGAGATTGCAACAAAATTGA
GACACGTTTGGCCTTGTTGCATTTTGAACGTTGCAAATTCATATTTGTTGCTAGTCGCTG
GGTTTGTTAAGCGGTTTAATTTTGAGTTCAAACCGAAAACAGGTTTAAGGAATGTGGTTT
TGAGAAAGGATGGGGAGAAAGCGCAGGGAGAGTAAAGGAGACAATGAGAATCTTGTCGGA
GATTCTTCAAGCCCCTGATCCTCAAAACATTGATCGATTCTTCGCAAGGGTTCCTAGAAT
CTTTAACGTTGTCATCTTCTCGGTTCACGGCTACTTTGGTCAAACCGATGTTCTAGGTTT
GCCTGATACTGGCGGTCAAGTCGTTTACA
   
Sequence Name 04-K016382-022-022-D04C-MS12
GenBank Accession LN487710 [GenBank]
Sequencing primer gene specific primer, MS12: 5'-TTGGTCAAGAATGTAAACGACTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K016382-022-022-D04C-ms12
CGCCAGTATCAGGCAAACCTAGAACATCGGTTTGACCAAAGTAGCCGTGAACCGAGAAGA
TGACAACGTTAAAGATTCTAGGAACCCTTGCGAAGAATCGATCAATGTTTTGAGGATCAG
GGGCTTGAAGAATCTCCGACAAGATTCTCATTGTCTCCTTTACTCTCCCTGCGCTTTCTC
CCCATCCTTTCTCAAAACCACATTCCTTAAACCTGTTTTCGGTTTGAACTCAAAATTAAA
CCGCTTAACAAACCCAGCGACTAGCAACAAATATGAATTTGCAACGTTCAAAATGCAACA
AGGCCAAACGTGTCTCAATTTTGTTGCAATCTCACGACTTGCTGAGTTACTGCAACAAAA
CATATTGTGGTAATATCACTATACGATATAGCAATGTAAATTATTAAAGGTTTAACCTGA
GTTCAAAGGCTTGGAAAGGTGTGTCCTTAGGCAGCTCCGATAGGAAAACATCTGCCAAAA
TCAAACTCATCTCAAGCTTTCCCGGGCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCTAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA
Primers used for PCR FL33 - 8474, show primer details
   
Sequence Name 03-K029742-022-022-D04C-8409
GenBank Accession LN487354 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K029742-022-022-D04C-8409
ATCATCTCAAGGTAAGAGTCTCATTCATCATAAGCTTCTGAAAATTATATAACAACATTG
AGATAGAACTGATTTAGGCTACAATGTCTTACAAACTTAAATTGTTTTACAAATTTAAAT
TTAAGCAAATACTATTATTATAAAAGGAAAATAAATGTTTGGGAAATTATTTTAAGAAAT
GCTACATAATACATTTGGTATTCCAAAACTAACAATCCTAAAGTTTATGAAATGTTCCCA
TTACTAGGGTCGTATATGTAGTACTACTTCACATCTTTATAATATTTTCTAAATAGGAGT
ATTTTAAATGAAAATTTTGAATTTTTGGTACATTAGATATCTATCTATATTAATGTCAAC
AGAAATCTAAGGTTTTATTTTCTTAATTCTTTTCATACGAAAACACAAATACGAAAACGT
CATTTACACAAGGAAACAAAAAACAATGAAGAAATGGATACGATGGGGACAAAACTAACC
TCTCCTTGGATGCT
   
Sequence Name 04-K029742-022-022-D04C-FL33
GenBank Accession LN487789 [GenBank]
Sequencing primer gene specific primer, FL33: 5'-TGAGCATCAAGGAGAGGTTAGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K029742-022-022-D04C-FL33
GTTTTTTGTTTCCTTGTGTAAATGACGTTTTCGTATTTGTGTTTTCGTATGAAAAGAATT
AAGAAAATAAAACCTTAGATTTCTGTTGACATTAATATAGATAGATATCTAATGTACCAA
AAATTCAAAATTTTCATTTAAAATACTCCTATTTAGAAAATATTATAAAGATGTGAAGTA
GTACTACATATACGACCCTAGTAATGGGAACATTTCATAAACTTTAGGATTGTTAGTTTT
GGAATACCAAATGTATTATGTAGCATTTCTTAAAATAATTTCCCAAACATTTATTTTCCT
TTTATAATAATAGTATTTGCTTAAATTTAAATTTGTAAAACAATTTAAGTTTGTAAGACA
TTGTAGCCTAAATCAGTTCTATCTCAATGTTGTTATATAATTTTCAGAAGCTTATGATGA
ATGAGACTCTTAACCTTGAGATGGATCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
TTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11