Sequences from confirmation amplicon


Confirmation sequences for line 030F04 and hit At5g48410 are shown.
Line ID 030F04
Locus At5g48410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19621787
Primers used for PCR MF29 - 8474, show primer details
   
Sequence Name 60-K031691-022-030-F04C-8409
GenBank Accession LN510163 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K031691-022-030-F04C-8409
CTTTACATTGAATATATTCGGTTGGATCAGTCTCCACTTTCATCAGTCTTGGGAATCTAT
TGCTAGATGTAACCAAGACCCTCAGCTTTTTTCTATCGCTATCTATTAGACTAGTCCCTT
TCGGACTTTGAGCAGACCCACCTGGCCAGATTATGGTCTCAAGCTTGTTATGAGTAGAAG
ATAAATGTCTTCTATTACTAAAACTACCGTTAGAGTTCAAGAATCCTACTCTTCTCTCAC
TTGATCCTATCATATTGATAATCTCAAACTTGTTTGATAACAACTTCTTATCATTTAGTT
GGAAATCACCACTCAAACCGTTAAACCTAGTCTTTGTGATTTCCTCAAGGAGAGT
   
Sequence Name 12-K031691-022-030-F04C-MF29
GenBank Accession LN491454 [GenBank]
Sequencing primer gene specific primer, MF29: 5'-AACTCTCCTTGAGGAAATCACAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K031691-022-030-F04C-MF29
ACTAAATGATAAGAAGTTGTTATCAAACAAGTTTGAGATTATCAATATGATAGGATCAAG
TGAGAGAAGAGTAGGATTCTTGAACTCTAACGGTAGTTTTAGTAATAGAAGACATTTATC
TTCTACTCATAACAAGCTTGAGACCATAATCTGGCCAGGTGGGTCTGCTCAAAGTCCGAA
AGGGACTAGTCTAATAGATAGCGATAGAAAAAAGCTGAGGGTCTTGGTTACATCTAGCAA
TAGATTCCCAAGACTGATGAAAGTGGAGACTGATCCAACCGAATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACG
TCCAAATGG

Last Updated on 06.12.2018 16:11