Sequences from confirmation amplicon


Confirmation sequences for line 030F05 and hit At4g15870 are shown.
Line ID 030F05
Locus At4g15870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9009079
Primers used for PCR 86mm - 8474, show primer details
   
Sequence Name 53-K114263-0022-030-F05C-86mm
GenBank Accession LN508226 [GenBank]
Sequencing primer gene specific primer, 86mm: 5'-CAGACCTATAAGTGAACTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K114263-0022-030-F05C-86mm
CGCGGCACACTCTTTGTTGTCTTTATATCTCCTCGTTCACAGTTGTATCCAAGTCCCTAG
CCATCTTTTGAAGCCTTGTGAAAGTTTCCTCCTCGGTAACTTTGTATTGCTTCATATAGT
ACATGATGGCATTAACATCAAACCCTCTGCTCATGTCACCCTGTTAGATATTACTAGTCA
TGTTTAACGTATAATGTCTTGATCACTAACCAAACAAGAAGAAACGCTTGATCATACAAT
TTTTGGTACCTCAAAGCCGGCTATGTCATTCATTAGACGAGCCCTTTTAGCCTGAGCCTC
AACAAATTTTGGTCTGGATTTTAGCCATTTATAAGCTTGTTCCCTAGAACTTTGTCCCAA
CCCAAGAAAACTGCATGCTATACTTACATACATTGTTACTTCGACTCCTCCNATCTCCAA
GTACTCGTCAAATGTAGGCACCACGTCTCCTTGTTCCCACTTGGTAAAGCAAAAGTTGGA
TCTNAAGTATACCTTGAACTGCTTATTAGGAGCATATAGAAGCACGTTAAACAACATCTA
ACTATNTAATTTTTTGATATTTGGATGTTACTTATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCACAAATGTAAATATCCGCA
   
Sequence Name 10-K114262-0022-030-F05C-8409
GenBank Accession LN490755 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K114262-0022-030-F05C-8409
GTAACATCCAAATATCAAAAAATTAAATAGTTAGATGTTGTTTAACGTGCTTCTATATGC
TCCTAATAAGCAGTTCAAGGTATACTTAAGATCCAACTTTTGCTTTACCAAGTGGGAACA
AGGAGACGTGGTGCCTACATTTGACGAGTACTTGGAGATTGGAGGAGTCGAAGTAACAAT
GTATGTAAGTATAGCATGCAGTTTTCTTGGGTTGGGACAAAGTTCTAGGGAACAAGCTTA
TAAATGGCTAAAATCCAGACCAAAATTTGTTGAGGCTCAGGCTAAAAGGGCTCGTCTAAT
GAATGACATAGCCGGCTTTGAGGTACCAAAAATTGTATGATCAAGCGTTTCTTCTTGTTT
GGTTAGTGATCAAGACATTATACGTTAAACATGACTAGTAATATCTAACAGGGTGACATG
AGCAGAGGGTTTGATGTTAATGCCATCATGTACTATATGAAGCAATACAAAGTTACCGAG
GAGGAAACTTTCACAAGGCTTCAAAAGATGGCTAGGGACTTGGATACAACTGTGAACGAG
GAGATATTAAAGACAACAAAGAGTGTGCCGCGGCAAATTCTAAAGCGGGCCATTGATTTT
GGAAAAATGATAGAGTTC

Last Updated on 06.12.2018 16:11