Sequences from confirmation amplicon


Confirmation sequences for line 044H01 and hit At4g29900 are shown.
Line ID 044H01
Locus At4g29900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14613685
Primers used for PCR IN32 - 8474, show primer details
   
Sequence Name 32-K033541-022-044-H01C-8409
GenBank Accession LN500479 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K033541-022-044-H01C-8409
GTATTCCATCTAGAGAGTTGCTCTTCATCAGTTGGAATCTTATCCGCTTCAAATGTTCGG
AAGGCAATTGCAACACAGCGCAGACTCCTTGCAGCCATATCATCGATAGCATCTTTTAAC
CCACCCATCTGAAACAACAAGACTTATACTCAGAATAAAAAACAAGTATTTCTATCAAGA
CTCTCATTACCAAATCTGGCACTGATTTGGACTTGCAGTATGACATGGTCTTGAGTCAAG
ACACAATGATAAGTTCAACATTCAGACAAACTCTATCATGATAAAGAGAAACATAGTTAC
TGACCTTATCTTCACTCATATCTACAAAACTCTCACTCTCATCCATATAGTGCGTGCAGG
ATCCCAAAACAATTTCGGCAGCACCTTTCCAGTGAATATGGACACTCGAATCTGGCTGAA
AATGAATTAGAAAGTCGTTAGGATGCTGATTGACAAGTAGAAACAACGATAAGAGAAAAA
TCAAAAACAAAGCAAAAGATCTACAAAAACTATGGGAGGAAAATGAA
   
Sequence Name 32-K033541-022-044-H01C-IN32
GenBank Accession LN500480 [GenBank]
Sequencing primer gene specific primer, IN32: 5'-TTTCATTTCCTCCCATAGTTTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K033541-022-044-H01C-IN32
TGATTTTCTCTTACGTTGTTTCTACTTGTCATCAGCATCCTAACGACTTTCTAATTCATT
TTCAGCCAGATTCGAGTGTCCATATTCACTGGAAAGGTGCTGCCGAAATTGTTTTGGGAT
CCTGCACGCACTATATGGATGAGAGTGAGAGTTTTGTAGATATGAGTGAAGATAAGGTCA
GTAACTATGTTTCTCTTTATCATGATAGAGTTTGTCTGAATGTTGAACTTATCATTGTGT
CTTGACTCAAGACCATGTCATACTGCAAGTCCAAATCAGTGCCAGATTTGGTAATGAGAG
TCTTGATAGAAATACTTGTTTTTTATTCTGAGTATAAGTCTTGTTGTTTCAGATGGGTGG
GTTAAAAGATGCTATCGATGATATGGCTGCAAGGAGTCTGCGCTGTGTTGCAATTGCCTT
CCGAACATTTGAAGCGGATAAGATTCCAACTGATGAAGAGCAACTCTCTAGATGGGAATT
ACCTGAGGATGATCAATACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTAATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11