Sequences from confirmation amplicon


Confirmation sequences for line 051A04 and hit At2g40460 are shown.
Line ID 051A04
Locus At2g40460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16897807
Primers used for PCR RK20 - 8474, show primer details
   
Sequence Name 55-K015974-022-051-A04C-8409
GenBank Accession LN508646 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K015974-022-051-A04C-8409
CATTTCCCGGACATCAAGCCATTTACAATTGAATATATCATACATAATGTAGACTCTGCT
CTTCAATTGAATATACTTTAATAAGCTTAAGAAAAAAAAATAGGTCATACATAATGTAGA
CTCTGCTCTTATTAATTACTGATATCATTTTCGTATAATTATATTTCACCAGTTCAGATT
TTTGAACTATTTTTAAATTAATATATTTTGTTTGGTTCATTTCTAAGTTCGATTTCTTCA
AAAAGTTGTAAAAGTCTGGTTTAGTTGAGTTTAATTCGTATTTTAACCATAAGAAATAAA
AGAGACTGTAGAATTAATACCAGGACGTAGATGAGAGAGGAGGCAGTAAAAGTCCAGAAG
CGACCGATGTATGAGTCAGCGATGTAAGCTCCGGCTATGGGAGTGATCCAAACCGCACCG
GACCAATTATTCACATTTCTAACCGAAGAGATCGTATCTTCGTGAAGTCTTTTAGTCAAA
TAATTCACTAAGTTCGACGCTATTCCATAAAACGCCATCCTCTCAAACGCTTCATACCCT
TCTCACACACACACACACAAAGACACAA
   
Sequence Name 56-K015974-022-051-A04C-RK20
GenBank Accession LN508958 [GenBank]
Sequencing primer gene specific primer, RK20: 5'-TTTTGTGTCTTTGTGTGTGTGTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K015974-022-051-A04C-rk20
GATGGCGTTTTATGGAATAGCGTCGAACTTAGTGAATTATTTGACTAAAAGACTTCACGA
AGATACGATCTCTTCGGTTAGAAATGTGAATAATTGGTCCGGTGCGGTTTGGATCACTCC
CATAGCCGGAGCTTACATCGCTGACTCATACATCGGTCGCTTCTGGACTTTTACTGCCTC
CTCTCTCATCTACGTCCTGGTATTAATTCTACAGTCTCTTTTATTTCTTATGGTTAAAAT
ACGAATTAAACTCAACTAAACCAGACTTTTACAACTTTTTGAAGAAATCGAACTTAGAAA
TGAACCAAACAAAATATATTAATTTAAAAATAGTTCAAAAATCTGAACTGGTGAAATATA
ATTATACGAAAATGATATCAGTAATTAATAAGAGCAGAGTCTACATTATGTATGACCTAT
TTTTTTTTCTTAAGCTTATTAAAGTATATTCAATTGAAGAGCAGAGTCTACATTATGTAT
GATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGA
TCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTT
CGACGTGTCTAC

Last Updated on 06.12.2018 16:11