Sequences from confirmation amplicon


Confirmation sequences for line 051C11 and hit At2g38910 are shown.
Line ID 051C11
Locus At2g38910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16245580
Primers used for PCR DE02 - 8474, show primer details
   
Sequence Name 07-K019240-022-051-C11C-8409
GenBank Accession LN489054 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K019240-022-051-C11C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATATCCGCCTATCCGCCTATTT
ACAAATGGTCTTCTTTCTCTATTTTGTTCAAATGATCCATAGTCTATTGTCCCACTGTTA
TCTATATCCGCCTGATCAAATGTTCATATTTTTCCAAATTATTATCTCACACATCTCACA
TCATATAGTAAACAAAAGGTGATTTCAGTATAAAAATGGACTTACTGCTTGCATTAATCC
TAGTATTTCTGAATCTTTAAGGTCAGCACCAACTCTGTCCAAACCTTTCTTGAGTTCTTC
GAGAGTGATGTGTCCACTATTATCTGTATCTATCATCTTGAACATTTCCTTCAATCCTGC
TATTTCTTCCTCTGACAAGCTTTCTGCTATTACCTGAATCAAATACATCACGTATAACTC
TCTATCTTGCACATAATTTGGTATAGATGAT
   
Sequence Name 08-K019240-022-051-C11C-DE02
GenBank Accession LN489523 [GenBank]
Sequencing primer gene specific primer, DE02: 5'-ATAAACCTCTTGATTCTGCTGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K019240-022-051-C11C-DE02
CAACAATTTTCAGCCATGAACAAGCTCAAGAAAATAGCTATCAAGGTAAACATTTTGAAT
TGGTTTCTATCATCTATACCAAATTATGTGCAAGATAGAGAGTTATACGTGATGTATTTG
ATTCAGGTAATAGCAGAAAGCTTGTCAGAGGAAGAAATAGCAGGATTGAAGGAAATGTTC
AAGATGATAGATACAGATAATAGTGGACACATCACTCTCGAAGAACTCAAGAAAGGTTTG
GACAGAGTTGGTGCTGACCTTAAAGATTCAGAAATACTAGGATTAATGCAAGCAGTAAGT
CCATTTTTATACTGAAATCACCTTTTGTTTACTATATGAT

Last Updated on 06.12.2018 16:11