Sequences from confirmation amplicon


Confirmation sequences for line 051E07 and hit At5g13440 are shown.
Line ID 051E07
Locus At5g13440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4309311
Primers used for PCR DL01 - 8474, show primer details
   
Sequence Name 12-K015112-022-051-E07C-DL01
GenBank Accession LN491348 [GenBank]
Sequencing primer gene specific primer, DL01: 5'-GTCTTAACCCATTTGAGCAGAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K015112-022-051-E07C-DL01
GCAGGAAGGAGGCTTTTTTCTGTTTCGCAGAGATCATCGACGGCGACCTCCTTCGTCCTT
TCCCGTGACCATACCCTTTCCGATGGAGGCAACTCCTCTTCCGCTTCTAGATCTGTCCCC
TCTGCAGATCTCTCATCTTTCAATTCTTACCACCGGAGCGTTATAAGAGGTCTGCCGATC
TACAACTCTTTCTGTCTCTCGATTGATTTTCATTTCTGGTGTTATCCATTGAATGATTCA
TAAATTTTTAAAAGTCACCTCTTTTGGGCTTGTATAAGTCCGGAAATGATTGAGATTCCT
AGTTTCGTATCTGCGAGATGATCTATGGGAATTAAGTCATCTGTTTTGAATTCTTATGAT
GTGTATTAGAGCTTGATGTACCATTTCGTCTCATTCTTCTCCTTGCTGGAATAGTGATTG
ATCGGATATGAATTGACCAGCTTTGAGATAATCCTTTTTTGATGAGATAATCGATTTTGA
CATGTAGTTTATTCATTATTATGGAAATGGATGTTTTATCGTCTTTGGGCACATCATATA
AGATACTTGCATCAGTAAACGAATCTGGAATTCCATATCTTTAACCAAAAGGGCTATGTT
TGCAATCTTATCTTANGATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATG
   
Sequence Name 11-K015112-022-051-E07C-8409
GenBank Accession LN490892 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K015112-022-051-E07C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGATCCTAAGATAAGAT
TGCAAACATAGCCCTTTTGGTTAAAGATATGGAATTCCAGATTCGTTTACTGATGCAAGT
ATCTTATATGATGTGCCCAAAGACGATAAAACATCCATTTCCATAATAATGAATAAACTA
CATGTCAAAATCGATTATCTCATCAAAAAAGGATTATCTCAAAGCTGGTCAATTCATATC
CGATCAATCACTATTCCAGCAAGGAGAAGAATGAGACGAAATGGTACATCAAGCTCTAAT
ACACATCATAAGAATTCAAAACAGATGACTTAATTCCCATAGATCATCTCGCAGATACGA
AACTAGGAATCTCAATCATTTCCGGACTTATACAAGCCCAAAAGAGGTGACTTTTAAAAA
TTTATGAATCATTCAATGGATAACACCAGAAATGAAAATCAATCGAGAGACAGAAAGAGT
TGTAGATCGGCAGACCTCTTATAACGCTCCGGTGGTAAGAATTGAAAGATGAGAGATCTG
CAGAGGGGACAGATCTAGAAGCGGAAGAGGAGTTGCCTCCATCGGAAAGGGTATGGTCAC
GGGA
Primers used for PCR FM11 - 8474, show primer details
   
Sequence Name 01-K029742-022-051-E07C-8409
GenBank Accession LN486445 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K029742-022-051-E07C-8409
ATTTACAATTGAATTTGATATCATGGTAGAAAATTGTTTGGATGACACCTTCTGTTATCT
CTTGGAATCCGTTTTTATAATCTTATGTTATTCTATGCATTAATGTTAAATGTTTGCTAG
TGTTATTCCTTTCTTCTGTGTTATCTCTTGAAAATATTTTTGCTTAATACTTGGTGTTGT
TGAACACTTGACCAAGTATAAAGTTTGCCCTTGATGTGAAATATTAATGTTTCATGCTAT
TTTCAGGTTTTGCTTCTCAAGTCATTACTCAGGGAAATGAGATAGGTTTTGGTTCGGAAG
TCCCAGCCACAGTCGAAGCTGTCAAAACACCTAACTCAAAGATTGTCTATGACGACCATA
ACCATGAGCGTTACCCACCTGGTGACCCTAGCAAGCGGGCATTCGCCTATTTTGTCTTGT
CGGGTGGAAGGTTTGTTTATGCCTCTGTTCTCCGCCTGCTTGTTCTGAAGCTTATTGTGA
GCATGTCTGCAAGTAAAGATGTCCTTGCCCTTGCATCCCTCGAGGTTGACCTAGGGAGCA
TAGAACCAGGAAC
   
Sequence Name 02-K029742-022-051-E07C-FM11
GenBank Accession LN486906 [GenBank]
Sequencing primer gene specific primer, FM11: 5'-TAGTTCCTGGTTCTATGCTCCCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K029742-022-051-E07C-FM11
TCTTTCTTGAACTCTCACAATAAGCTTCAGAACAAGCAGGCGGAGAACAGAGGCATAAAC
AAACCTTCCACCCGACAAGACAAAATAGGCGAATGCCCGCTTGCTAGGGTCACCAGGTGG
GTAACGCTCATGGTTATGGTCGTCATAGACAATCTTTGAGTTAGGTGTTTTGACAGCTTC
GACTGTGGCTGGGACTTCCGAACCAAAACCTATCTCATTTCCCTGAGTAATGACTTGAGA
AGCAAAACCTGAAAATAGCATGAAACATTAATATTTCACATCAAGGGCAAACTTTATACT
TGGTCAAGTGTTCAACAACACCAAGTATTAAGCAAAAATATTTTCAAGAGATAACACAGA
AGAAAGGAATAACACTAGCAAACATTTAACATTAATGCATAGAATAACATAAGATTATAA
AAACGGATTCCAAGAGATAACAGAAGGTGTCATCCAAACAATTTTCTACCATGATATCAA
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACG
TGTCTACA

Last Updated on 06.12.2018 16:11