Sequences from confirmation amplicon


Confirmation sequences for line 052B10 and hit At3g16980 are shown.
Line ID 052B10
Locus At3g16980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5795321
Primers used for PCR 10eq - 8474, show primer details
   
Sequence Name 30-K110907-0022-052-B10C-10eq
GenBank Accession LN499772 [GenBank]
Sequencing primer gene specific primer, 10eq: 5'-TGAATACGGATTTGTCTCTGACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K110907-0022-052-B10C-10eq
ACTTACTGGTAACTACATCAACTCCTAAAACTCTTAAGAACTCACCAATTGAAACCGAAA
AGCTCTTTATTCTCTCCAGCGATGACCACAATTCGGGTTGCAACAGACAAAGAACAGTGT
CATTCCTTCTTCACCTCTAGCCGTAGCCTGCACATTCAATGATAGCTACATTAAATCCTC
AAGCGGTGTACAGAATTTGAGCATAATGTTTTGGGGAAGCAAACAGTGAAGATACAGCAT
AACCAAGTAGTTAAATAAATAATGGAGCTTAAGAGAGTTATACCTGGAAGAAAACGGCCT
CCCTATGCTGACACTTAGAGCAACGCACAGCCTTGGTTCGGGGAAGAGTAGGGTCAGAAG
CCACGTCTGTTAAGATCTGAGTTCGCTCACTTACAGAGTGATGAACCTCGTTTCTGTACA
CACAGCTGTTATCAGCTACCTCCTGACAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 14-K110898-0022-052-B10C-8409
GenBank Accession LN492562 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K110898-0022-052-B10C-8409
ATTTACAATTGAATATATCCTGTCAGGAGGTAGCTGATAACAGCTGTGCTGTACAGAAAC
GAGGTTCATCACTCTGTAAGTGAGCGAACTCAGATCTTAACAGACGTGGCTTCTGACCCT
ACTCTTCCCCGAACCAAGGCTGTGCGTTGCTCTAAGTGTCAGCATAGGGAGGCCGTTTTC
TTCCAGGTATAACTCTCTTAAGCTCCATTATTTATTTAACTACTTGGTTATGCTGTATCT
TCACTGTTTGCTTCCCCAAAACATTATGCTCAAATTCTGTACACCGCTTGAGGATTTAAT
GTAGCTATCATTGAATGTGCAGGCTACGGCTAGAGGTGAAGAAGGAATGACACTGTTCTT
TGTCTGTTGCAACCCGAATTGTGGTCATCGCTGGAGAGAATAAAGAGCTTTTCGGTTTCA
ATTGGTGAGTTCTTAAGAGTTTTAGGAGTTGATGTAGTTACCAGTAAGTGTAATATGTGG
AATGACGGGAACAACCTTCTGCCCCAGTCAGAGACAATCCCNTATTC

Last Updated on 06.12.2018 16:11