Sequences from confirmation amplicon


Confirmation sequences for line 053B09 and hit At1g35670 are shown.
Line ID 053B09
Locus At1g35670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:13206540
Primers used for PCR DL49 - 8474, show primer details
   
Sequence Name 24-K018489-022-053-B09C-DL49
GenBank Accession LN496802 [GenBank]
Sequencing primer gene specific primer, DL49: 5'-CAACAGTTGCTAATTCCAAATGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K018489-022-053-B09C-DL49
CAACAAAGAATGAACATACACAAGGCTTCATGAGCAGAAATGCGTTTCTTGGGGCTCCTT
TCGAGCATTTTATAGATCAAATCTTTAGCAGCTTCTGAGATAGTAGGCCACGGGTCAGAT
TTGAAATCTAACTTCCCTTGCAATATCTGTCTAAAGATTCCAGACTCAGTCTCTGCAATG
CCCAAGAGAAAAGAATACATCATTCAAACAAAAACTCGAAACTTGTAAATAGAAAAGACT
CTACTTTTTTAGTAGACTAACCTGCCCAGAAGGGAGGAACACCGCTGAGTAAAATGTAGA
GGATAACACCAGCACTCCACACATCTATTTCAGGTCCATAACATTTCTTTAGCACCTCTG
GTGCAACATAGTACGGACTTCCAACTACGTCATATAAATATTGTCCTGAAGAAGCAAACA
AAAACCAATTAGCCGATAAACTGGCATTAATGAGAAGGTTCAAGCCAGACACACAAATGC
AGAATAATTTCGATCTACACATTAAGTTTACAATTAATCTACATGCAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATG
   
Sequence Name 28-K018489-022-053-B09C-8409
GenBank Accession LN498644 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K018489-022-053-B09C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCATGTAGATTAATTGT
AAACTTAATGTGTAGATCGAAATTATTCTGCATTTGTGTGTCTGGCTTGAACCTTCTCAT
TAATGCCAGTTTATCGGCTAATTGGTTTTTGTTTGCTTCTTCAGGACAATATTTATATGA
CGTAGTTGGAAGTCCGTACTATGTTGCACCAGAGGTGCTAAAGAAATGTTATGGACCTGA
AATAGATGTGTGGAGTGCTGGTGTTATCCTCTACATTTTACTCAGCGGTGTTCCTCCCTT
CTGGGCAGGTTAGTCTACTAAAAAAGTAGAGTCTTTTCTATTTACAAGTTTCGAGTTTTT
GTTTGAATGATGTATTCTTTTCTCTTGGGCATTGCAGAGACTGAGTCTGGAATCTTTAGA
CAGATATTGCAAGGGAAGTTAGATTTCAAATCTGACCCGTGGCCTACTATCTCAGAAGCT
GCTAAAGATTTGATCTATAAAATGCTCGAAAGGAGCCCCAAGAAACGCATTTCTGCTCAT
GAAGCCTTGTGTATGTTCATTCTTTGTTGTTCATTTGGA
Primers used for PCR FM13 - 8474, show primer details
   
Sequence Name 25-K029743-022-053-B09C-8409
GenBank Accession LN497311 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K029743-022-053-B09C-8409
GATCATACAATGTTCCCTCTAAGAAGGTAACAAATTTGAATCCAACCAAACAGATCATCA
AACCTATACATACCGAAAACCTTATTTCGATTTGCAATCCATATAAAACACAACAAACCT
GTTTCTATCAAGAAACCACTTTGTTTCAATTTTCGATTCATAGAAAACACAAATAACCTG
ATTCTACAAGAAACCAAACCAAAGACTTAATCAAATGCAGAATAGTTCCAATCCACACAT
TCAAAGACTCCTCAATGAGTAACAACTAACCAAAGCAAGCTCCCTAACTACACCTTATAG
ACATTGTCCTGAAGAGTAAACTAAAACCAATTAGGCGATGAACCACCAACAATAAGGTTT
CAAAGAA
   
Sequence Name 26-K029743-022-053-B09C-FM13
GenBank Accession LN497788 [GenBank]
Sequencing primer gene specific primer, FM13: 5'-CTTTGAAACCTTATTGTTGGTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K029743-022-053-B09C-FM13
TCTTCGGACATGTCTATAAGGTGTAGTTAGGGAGCTTGCTTTGGTCCTTCTAACTCATTG
AGGAGTCTTTGAATGTGTGGATTGGACCTATTATGCATTTGATTAAGTCTTTGGTTTGGT
TTCTTGTAGAATCAGGTTATTTGTGTTTTCTATGAATCGAAAATTGAAACAAAGTGGTTT
CTTGATAGAAACAGGTTTGTTGTGTTTTATATGGATTGCAAATCGAAATAAGGTTTTCGG
TATGTATAGGTTTGATGATCTGTTTGGTTGGATTCAAATTTGTTACCTTCTTAGAGGGAA
CATTGTATTGATCGAAATTAATGTGTATAGATGTTCGTCATTGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCATTTCAAAAATGTAGATGTCCGCAG
CGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTA
TAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA
TTCACGTCCAAAATGG

Last Updated on 06.12.2018 16:11