Sequences from confirmation amplicon


Confirmation sequences for line 064B02 and hit At3g63070 are shown.
Line ID 064B02
Locus At3g63070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23307514
Primers used for PCR 07sj - 8474, show primer details
   
Sequence Name 41-K116478-0022-064-B02C-07sj
GenBank Accession LN504218 [GenBank]
Sequencing primer gene specific primer, 07sj: 5'-CCATATTCATCCACCAGTATACCC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K116478-0022-064-B02C-07sj
AGCGGATAGCCGAGAATAGAGGCAAGCAGGAACATTACTAAGCGAATCAAGTTCTCTTAT
ATGGTGACGAACTATAGATTCAGGAAGGATCCGTCTTTCAAGCCAAAGCCTCAAAACCTA
TATAAGAGCAGAAAGGGTGAGAGTTAGTATTCATAAGAAAAAGAATATCTAACTAAAATC
GCATACCTTCAAGCACTGTTTTCGGTTTTCTTGTGTGGTAGCTCCAGCTGGGACAGCAGC
AGTTAATAGGCGAGGTAGCATAGCCTGAATGGATGAAAGATAAACACCACCAGCATCACC
TGAATAGAAAGGTAGTATTCACAAAGTTAACTCGCCATTGAGAACATCAATGATGCAACT
AAGCAAAAACAGCATACTCACCATTCAAACCTTTGGAGCACTGAGCAATAGAATCCACAA
GGAAAAATAAATCCACCCTCCTTTGTAAATTTGACTCACTTTCCAAAGTGTGGGCCAGCA
AAACAAAACATTAGACAAAGATCATGAAGCCTATCAGTTTTCTTATTGCCAGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CG
   
Sequence Name 16-K116477-0022-064-B02C-8409
GenBank Accession LN493585 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K116477-0022-064-B02C-8409
TTGAATTATCCTGGCAATAAGAAAACTGATAGGCTTCATGATCTTTGTCTAATGTTTTGT
TTTGCTGGCCCACACTTTGGAAAGTGAGTCAAATTTACAAAGGAGGGTGGATTTATTTTT
CCTTGTGGATTCTATTGCTCAGTGCTCCAAAGGTTTGAATGGTGAGTATGCTGTTTTTGC
TTAGTTGCATCATTGATGTTCTCAATGGCGAGTTAACTTTGTGAATACTACCTTTCTATT
CAGGTGATGCTGGTGGTGTTTATCTTTCATCCATTCAGGCTATGCTACCTCGCCTATTAA
CTGCTGCTGTCCCAGCTGGAGCTACCACACAAGAAAACCGAAAACAGTGCTTGAAGGTAT
GCGATTTTAGTTAGATATTCTTTTTCTTATGAATACTAACTCTCACCCTTTCTGCTCTTA
TATAGGTTTTGAGGCTTTGGCTTGAAAGACGGATCCTTCCTGAATCTATAGTTCGTCACC
ATATAAGAGAACTTGATTCGCTTAGTAATGTTCCTGCTTGCCTCTATTCTCGGCGATCCG
CTCGAACAGAAAGGGCGCTGGATGACCCTGTTAGAGATATGGAGGGTATACTGGTGATGA
AATATGG

Last Updated on 06.12.2018 16:11