Sequences from confirmation amplicon


Confirmation sequences for line 065A09 and hit At4g28210 are shown.
Line ID 065A09
Locus At4g28210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13992098
Primers used for PCR RK37 - 8474, show primer details
   
Sequence Name 49-K015906-022-065-A09C-8409
GenBank Accession LN506660 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K015906-022-065-A09C-8409
ATTAGATGGCTTCTAGCTAAGAAATAGCCCACCACCAGTTCTCGAAATGGGCCGTATTAA
CTAACATTATCCAAACAGAGAAACAAAACCTTTTTCTTGTATCATCAAAAATTTCTCTTC
AAACATTGGGGAAAGGTACAACAAGTTACAACACAAACTAGTTTTGTTCGTCTCTAAAGT
TCTTTGTTTCAGCAGAGAATCAAACAATCATCCAAGTCAAGTGTCTGTATCCAGCCCATT
TTGCAGCAAGTCGGACGCCAATGGCTAATGCTGCAGCCAACAACGGCATTGGTGGTGCCC
TCCTTAAGATCTGACTCTCTTCTTCTGAGGAGGTCTTCGTTGAATTGTGATACATGGCTA
GAGCCAGATACCCTGTGATAGCCACCACTCTCACCCATGGCTCCCCTACTCCTGCCAGAA
CAGCTGCAGAAGGAAGAGCCGAGGTCACATCGTCTCTCTTCTTTATCAACCGCGAGTACC
CATACAAACCTAACAAGACAGTCCAGAAGAACGGCTCTGAGATATAACTCTGCACCGCTG
CATACCCCACCAGACCCGCAA
   
Sequence Name 50-K015906-022-065-A09C-RK37
GenBank Accession LN507010 [GenBank]
Sequencing primer gene specific primer, RK37: 5'-GTATGTGGTAACTATTGCGGGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K015906-022-065-A09C-rk37
GGTATGCAGCGGTGCAGAGTTATATCTCAGAGCCGTTCTTCTGGACTGTCTTGTTAGGTT
TGTATGGGTACTCGCGGTTGATAAAGAAGAGAGACGATGTGACCTCGGCTCTTCCTTCTG
CAGCTGTTCTGGCAGGAGTAGGGGAGCCATGGGTGAGAGTGGTGGCTATCACAGGGTATC
TGGCTCTAGCCATGTATCACAATTCAACGAAGACCTCCTCAGAAGAAGAGAGTCAGATCT
TAAGGAGGGCACCACCAATGCCGTTGTTGGCTGCAGCATTAGCCATTGGCGTCCGACTTG
CTGCAAAATGGGCTGGATACAGACACTTGACTTGGATGATTGTTTGATTCTCTGCTGAAA
CAAAGAACTTTAGAGACGAACAAAACTAGTTTGTGTTGTAACTTGTTGTACCTTTCCCCA
ATGTTTGAAGAGAAATTTTTGATGATACAAGAAAAAGGTTTTGTTTCTCTGTTTGGATAA
TGTTAGTTAATACGGCCCATTTCGAGAACTGGTGGTGGGCTATTTCTTAGCTAGAAGCCA
TCTAACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGANAGCAATAAACAAATT
ATTCTAATTCGGAAATCT

Last Updated on 06.12.2018 16:11