Sequences from confirmation amplicon


Confirmation sequences for line 068A09 and hit At2g13620 are shown.
Line ID 068A09
Locus At2g13620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:5679901
Primers used for PCR KM38 - 8474, show primer details
   
Sequence Name 76-K032613-022-068-A09C-8409
GenBank Accession LN513327 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K032613-022-068-A09C-8409
AAGCCATTACCTACNCGTTAGTCAACCAAAACCTACTCGAAAACTCGCCTTGTTCGGTCG
GAATACTCGTGGATAGAGGACTCAATGGAGCGACAAGACTCAACTCAAACACTGTTTCTC
TTCAAGTTGCTGTTCTGTTTTTCGGCGGTCCAGACGATAGAGAAGCTTTGGCTTATGCAT
GGAGAATGGCTCAACATCCTGGCATCACTCTAACTGTTTTACGGTTCATTCATGATGAAG
ATGAAGCAGACACAGCTTCAACACGAGCGACCAATGACAGTGACCTAAAGATACCAAAGA
TGGACCATAGAAAACAAAGACAACTTGATGATGACTACATTAATCTCTTTAGGGCAGAAA
ATGCGGAATACGAGTCAATTGTGTATATAGAAAAACTAGTGAGCAATGGGGAAGAGACTG
TTGCAGCAGTGAGATCAATGGACAGTTCTCATGATTTGTTTATAGTTGGAAGAGGAGAAG
GAATGTCGTCTCCTCTTACCGCGGGTTTAACTGATTGGAGTGAGTGTCCTGAGCTTGGTG
CCATTGGAGATTTGCTTGCTTCATC
   
Sequence Name 76-K032613-022-068-A09C-KM38
GenBank Accession LN513328 [GenBank]
Sequencing primer gene specific primer, KM38: 5'-ACAGTAGCTGCAAAGTCTGATGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K032613-022-068-A09C-KM38
GCTCAGACACTCACTCCATCAGTTAAACCCGCGGTAAGAGGAGACGACATTCCTTCTCCT
CTTCCAACTATAAACAAATCATGAGAACTGTCCATTGATCTCACTGCTGCAACAGTCTCT
TCCCCATTGCTCACTAGTTTTTCTATATACACAATTGACTCGTATTCCGCATTTTCTGCC
CTAAAGAGATTAATGTAGTCATCATCAAGTTGTCTTTGTTTTCTATGGTCCATCTTTGGT
ATCTTTAGGTCACTGTCATTGGTCGCTCGTGTTGAAGCTGTGTCTGCTTCATCTTCATCA
TGAATGAACCGTAAAACAGTTAGAGTGATGCCAGGATGTTGAGCCATTCTCCATGCATAA
GCCAAAGCTTCTCTATCGTCTGGACCGCCGAAAAACAGAACAGCAACTTGAAGAGAAACA
GTGTTTGAGTTGAGTCTTGTCGCTCCATTGAGTCCTCTATCCACGAGTATTCCGACCGAA
CAAGGCGAGTTTTCGAGTAGGTTTTGGTTGACTAAACGGTAGGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
Primers used for PCR FP08 - 8474, show primer details
   
Sequence Name 04-K035479-022-068-A09C-FP08
GenBank Accession LN487821 [GenBank]
Sequencing primer gene specific primer, FP08: 5'-CCTTTACAAGCCAGTGAAGAAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K035479-022-068-A09C-FP8
ACATCCAACAACGAAGCCAGACAGCGAGCTTCGGGTATTGGTTTGCGTTCACACACCGCG
TAATGTTCCTACTATAATTAACCTTCTTGAAGCTTCACATCCCACAAAGAGATCTCCTAT
ATGCATCTACGTTCTCCACCTTGTTGAGCTCACGGGCCGCGCATCCGCGATGTTGATTGT
CCACAACACTCGTAAGTCAGGACGACCCGCGCTTAACAGAACTCAAGCTCAATCAGATCA
TATCATCAACGCCTTTGAGAACTATGAACAACACGCTGCCTTTGTCGCTGTACAACCTTT
AACAGCTATTTCACCTTACTCAACAATGCATGAAGATGTTTGTAGCTTAGCAGAGGACAA
ACGTGTCTCCTTCATAATCATACCATTTCACAAACAACAAACAGTTGATGGACAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACG
TGTCTACATTC
   
Sequence Name 03-K035479-022-068-A09C-8409
GenBank Accession LN487386 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K035479-022-068-A09C-8409
GAGCCATTTAAATTGAATATATCCTGTCCATCAACTGTTTGTTGTTTGTGAAATGGTATG
ATTATGAAGGAGACACGTTTGTCCTCTGCTAAGCTACAAACATCTTCATGCATTGTTGAG
TAAGGTGAAATAGCTGTTAAAGGTTGTACAGCGACAAAGGCAGCGTGTTGTTCATAGTTC
TCAAAGGCGTTGATGATATGATCTGATTGAGCTTGAGTTCTGTTAAGCGCGGGTCGTCCT
GACTTACGAGTGTTGTGGACAATCAACATCGCGGATGCGCGGCCCGTGAGCTCAACAAGG
TGGAGAACGTAGATGCATATAGGAGATCTCTTTGTGGGATGTGAAGCTTCAAGAAGGTTA
ATTATAGTAGGAACATTACGCGGTGTGTGAACGCAAACCAATACCCGAAGCTCGCTGTCT
GGCTTCGTTTGTTGGATTGTTCGTCTCTTGTATGAAACAGATTTCTTCACTGGCTTGTAA
AG

Last Updated on 06.12.2018 16:11