Sequences from confirmation amplicon


Confirmation sequences for line 069C05 and hit At5g09960 are shown.
Line ID 069C05
Locus At5g09960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3107270
Primers used for PCR 04kk - 3144, show primer details
   
Sequence Name 43-K113595-0022-069-C05C-04kk
GenBank Accession LN504905 [GenBank]
Sequencing primer gene specific primer, 04kk: 5'-GAAAAAGAAAAATGGATTGGAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K113595-0022-069-C05C-04kk
TTACACATTTCCCATTCAATCACAAGTCAATAATAAAACTGCTACTTTGCTCAAGAAGAA
ATCTTCAAGCTTTAACGCAGCAGTTGAAGCAGCTGAACATACTTCTCCTCGTCTGTCAAA
AAAAACAACCACACCAAATTCTCATCTATTCCACAGTGATAATCACTACCAGCTCAAATA
CAACTATTCAAAATTTTAATACTGTATATAGATTGCAAGATTACAAAACCATGTGCTAAT
ATAGTAATATGCCTCTAAATGTCTTAATGATAGTAATGCAAATATAATTAAGGAATAATA
TGATATAAACCAAATAATGGTGAAGAATCTTACAGAAGGAGAATGTGGTTGATGATGGTG
GCTGGGAGAGAACTCTCGTTGGTTTTCACTTTGGACCGTGGTAAGCTCAGCAGTTGATGT
TGCTGACTCGGGTGGCTTAATGAGCTCTTCCTCGGTTCAAACACTGATAGTTTAAACCGA
AGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTC
CTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAG
TGGAGATATC
   
Sequence Name 01-K113594-0022-069-C05C-3144
GenBank Accession LN486653 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K113594-0022-069-C05C-3144
TTCGCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGG
GGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAACCGAGGAAGAG
CTCATTAAGCCACCCGAGTCAGCAACATCAACTGCTGAGCTTACCACGGTCCAAAGTGAA
AACCAACGAGAGTTCTCTCCCAGCCACCATCATCAACCACATTCTCCTTCTGTAAGATTC
TTCACCATTATTTGGTTTATATCATATTATTCCTTAATTATATTTGCATTACTATCATTA
AGACATTTAGAGGCATATTACTATATTAGCACATGGTTTTGTAATCTTGCAATCTATATA
CAGTATTAAAATTTTGAATAGTTGTATTTGAGCTGGTAGTGATTATCACTGTGGAATAGA
TGAGAATTTGGTGTGGTTGTTTTTTTTGACAGACGAGGAGAAGTATGTTCAGCTGCTTCA
ACTGCTGCGTTAAAGCTTGAAGATTTCTTCTTGAGCAAAGTAGCAGTTTTATTATTGACT
TGTGATTTGAATGTGGAAATGTGTTAATGTCATGACACTTTAATATATGTTCCAATCCAT
T

Last Updated on 06.12.2018 16:11