Sequences from confirmation amplicon


Confirmation sequences for line 073E12 and hit At4g12250 are shown.
Line ID 073E12
Locus At4g12250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7290309
Primers used for PCR MP05 - 8474, show primer details
   
Sequence Name 43-K017122-022-073-E12C-MP05
GenBank Accession LN504662 [GenBank]
Sequencing primer gene specific primer, MP05: 5'-ATTTTTGACTTGCTCAATTTTGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K017122-022-073-E12C-MP05
CCAAAAATCCAAACCTAACCCTAATCGACATTTTCTCCGATTTCTAAATCCTCCTCCGGT
GAAAATGTCTCACCTTGATGATCTTCCTTCTACTCCCGGAAAGTACAAAACCGATAAAGT
TCCACCGTACGGGATTCTCCACCACCACCGTTACCTCCGTCTCTCAAAACTCACCCTTTG
GGCTTCTCTCTTCCTCGCTCTCTTCCTCTTCTACCTCGTTTTATCTCCTCCTCCGTCACC
ATCTCGCCGGAATCTCAACGATTCTTCCTCCATTTCCGCCGCGAAATACGGCGGCTCTCA
CTGGGAGAAACAAGTCCGTAAATCCGCTCGTCCTAGGTCTCACGGCGGTTTAACGGTATT
AGTCACCGGAGCTTCCGGATTCGTCGGTACTCACGTCTCGATCGCGTTACGAAGACGCGG
CGATGGTGTTCTAGGTCTCGACAATTTCAATCGATACTACGATCCAAAGCTTAAACGAGC
AAGACAAGGCCTTTTAGAAAGATCAGGTGTTTTCGTAGTCGAAGGCGATATAAACGACGC
CGTTTTACTCAGAAAGCTCTTTGATGTTGTCCTCTTCACACATGTTATGCATCTCGCTGC
TCAATTGGACATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATA
   
Sequence Name 44-K017122-022-073-E12C-8409
GenBank Accession LN505022 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K017122-022-073-E12C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATGTCCAATTGAGCAGCGAGATGC
ATAACATGTGTGAAGAGGACAACATCAAAGAGCTTTCTGAGTAAAACGGCGTCGTTTATA
TCGCCTTCGACTACGAAAACACCTGATCTTTCTAAAAGGCCTTGTCTTGCTCGTTTAAGC
TTTGGATCGTAGTATCGATTGAAATTGTCGAGACCTAGAACACCATCGCCGCGTCTTCGT
AACGCGATCGAGACGTGAGTACCGACGAATCCGGAAGCTCCGGTGACTAATACCGTTAAA
CCGCCGTGAGACCTAGGACGAGCGGATTTACGGACTTGTTTCTCCCAGTGAGAGCCGCCG
TATTTCGCGGCGGAAATGGAGGAAGAATCGTTGAGATTCCGGCGAGATGGTGACGGAGGA
GGAGATAAAACGAGGTAGAAGAGGAAGAGAGCGAGGAAGAGAGAAGCCCAAAGGGTGAGT
TTTGAGAGACGGAGGTAACGGTGGTGGTGGAGAATCCCGTACGGTGGAACTTTATCGGTT
TTGTACTTTCCGGGAGTAGAAGGAAGATCATCAAGGTGAGACATTTTCACCGGAGGAGGA
TTTAGAAATCGGAGAAAATGTCGATTAGGGTTAGGTTTGGATTTTTGGGTCTTAAGGAAG
CAAAATT

Last Updated on 06.12.2018 16:11