Sequences from confirmation amplicon


Confirmation sequences for line 076B06 and hit At3g01516 are shown.
Line ID 076B06
Locus At3g01516 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:207405
Primers used for PCR 05wc - 8474, show primer details
   
Sequence Name 16-K118356-0022-076-B06C-05wc
GenBank Accession LN493627 [GenBank]
Sequencing primer gene specific primer, 05wc: 5'-AAAGATTCAGATTCATATCCCACG-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K118356-0022-076-B06C-05wc
TCAACCAATCTCAATTTGAAAATTGAAATAGACAAAATCGTGACATTGGGACATAAAAAA
GCACTAGGACAAAATTCTAGTCGACGCAAAAAAAATTCGAAAAACGTGAGTCAGCGTATG
TGGTTTACACGCGCCAAAGTGGGAGCGTGAGTGGGGTTTGAATGCACCTGAAACTGTCTG
GTCAATAAGTTAAAGTGCATGGGAAAAATCTTGGTTGCTGTTTCCTATATATCTCTCTTT
TTACCATCAACATGTTACATATATATATGGACAAAAATCTGTATATGTGAATGCATATAG
GAGTGTGATGTATTTATATAAGTTAGTAAGCTTTGTTCTCTTTCTCTATTTTTTTGGAAA
ATTGGTAACGATGCAATTTTATATATAGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 16-K118355-0022-076-B06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K118355-0022-076-B06C-8409
TGGTTGCTTGGGAGAGCGGGAAAGAGTGGGGGCCCCCCCCCCC

Last Updated on 06.12.2018 16:11