Sequences from confirmation amplicon


Confirmation sequences for line 080A05 and hit At4g27530 are shown.
Line ID 080A05
Locus At4g27530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13752950
Primers used for PCR 03qz - 8474, show primer details
   
Sequence Name 60-K115869-0022-080-A05C-03qz
GenBank Accession LN510341 [GenBank]
Sequencing primer gene specific primer, 03qz: 5'-CTCGATCCTCAAAGTACTTCCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K115869-0022-080-A05C-03qz
ATATGAATTGAAAGTGTAGAAAATGGCGGCTATGTTTTTAGCAAAGAGAGGAGCCATAAA
CATACATAGAGCTTCTTCATTACACATAGATTACAACAACAGTTCTCGATCTGTTGTTGA
TGGAGTCATTACTATCACTAACTCTCGTTACTTCGCATCCCACGTACGTTTTTGAAATTT
GTTGAAGATAGATTAATTATGCATACAAAAATCGTTGAGCTGTTTTCACTTAATATTTTC
ACATATTCTGCAGGGGAACAACGGAGAGAAGTCAGAGGAAGAGGCGATGGGGACAACTAC
AGATGGTAAAGCTCCGAATGTGACATTGGGTCAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 19-K115867-0022-080-A05C-8409
GenBank Accession LN494904 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K115867-0022-080-A05C-8409
CATTACATTGAATATATCCTGACCCAATGTCACATTCGGAGCTTTACCATCTGTAGTTGT
CCCCATCGCCTCTTCCTCTGACTTCTCTCCGTTGTTCCCCTGCAGAATATGTGAAAATAT
TAAGTGAAAACAGCTCAACGATTTTTGTATGCATAATTAATCTATCTTCAACAAATTTCA
AAAACGTACGTGGGATGCGAAGTAACGAGAGTTAGTGATAGTAATGACTCCATCAACAAC
AGATCGAGAACTGTTGTTGTAATCTATGTGTAATGAAGAAGCTCTATGTATGTTTATGGC
TCCTCTCTTTGCTAAAAACATAGCCGCCATTTTCTACACTTTTCAATTCATTATCTAGCT
CTCGCTCTCATCTATATACACACCGATGGAAGTACTTGGGGGATCGA

Last Updated on 06.12.2018 16:11