Sequences from confirmation amplicon


Confirmation sequences for line 080G06 and hit At1g05570 are shown.
Line ID 080G06
Locus At1g05570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1647926
Primers used for PCR VL21 - 8474, show primer details
   
Sequence Name 05-K021056-022-080-G06C-8409
GenBank Accession LN488165 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K021056-022-080-G06C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGCATAGCCCATTTTCTTGGTGGTC
AAAGAAAAGACCGGTCTTCAAAAAACAAGGAGTGACATGTACACATGTAAATGGCGTATG
TGTAGATTTAATGGTTATCTGTTATTGTTTCCTTCCCAAGTAATCTATAGTTAGAAAGTT
CCTGTAACTTTCATAGATATATGTTAATTATTTTCCATTTCCATAAGTTCGCGTCCATTA
TAATAGAAAGTGCATGAGACGTATCTTTTTCATATGGAAGACGAAAACATTGGTTCTCCA
TTCTAAAAGTCAAAAAGAAAATGTATATCACTTGAACTTTTCCACATAGGTCATGATAAA
GACAGGACTAATCAATCATTCATTGTTGATAATGGTCTCAATTACCTGTTGCTAGTTATT
TCTACATTTGTATAGTGCAATACTGTTTTTTTTTCCTCTTCCAAATAAAAACTACAGTAC
TAAAAGAAAGTTGGTAATCATAAAAGTTATAAAACTCAAATAAAAGCAATTACTCTGTTT
CTTTTTCACTCTTATTTTATATGACTGAAAT
   
Sequence Name 06-K021056-022-080-G06C-VL21
GenBank Accession LN488612 [GenBank]
Sequencing primer gene specific primer, VL21: 5'-TAAACGCTTCATCGTATAAATCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K021056-022-080-G06C-VL21
CGGTTGTTTTTTGCAGATTTGATGTTTACATATCAATGAACATACAAACAAAAACAACAT
ATAGACAATTTCATTTCAGTCATATAAAATAAGAGTGAAAAAGAAACAGAGTAATTGCTT
TTATTTGAGTTTTATAACTTTTATGATTACCAACTTTCTTTTAGTACTGTAGTTTTTATT
TGGAAGAGGAAAAAAAAACAGTATTGCACTATACAAATGTAGAAATAACTAGCAACAGGT
AATTGAGACCATTATCAACAATGAATGATTGATTAGTCCTGTCTTTATCATGACCTATGT
GGAAAAGTTCAAGTGATATACATTTTCTTTTTGACTTTTAGAATGGAGAACCAATGTTTT
CGTCTTCCATATGAAAAAGATACGTCTCATGCACTTTCTATTATAATGGACGCGAACTTT
TGGAAATGGAAAATAATTAACATATATCTATGAAAGTTACAGGAACTTTCTAACTATAGA
TTACTTGGGAAGGAAACAATAACAGATAACCATTA

Last Updated on 06.12.2018 16:11