Sequences from confirmation amplicon


Confirmation sequences for line 083C06 and hit At5g15070 are shown.
Line ID 083C06
Locus At5g15070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4879581
Primers used for PCR 08sj - 8474, show primer details
   
Sequence Name 28-K116518-0022-083-C06C-08sj
GenBank Accession LN499003 [GenBank]
Sequencing primer gene specific primer, 08sj: 5'-ATTCTGTTTTAATCCATGACCAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K116518-0022-083-C06C-08sj
TGTTTTATGCTCTTGTTTATCCGGTCTCATAAATTGAGATAGTTGCCGTGTTAATGTCCT
TATGCTGTTTTGTTGTCCTGTTTGTTCCTGCAATGGATATTTTTCCATGTCGTAAGGCTA
TCTGGAATAGTTTTTTTTTTCTGGATTATGTTTTGGTTGAAGCTTGGCTTTGACAAGTTT
TTGACTGACAGTCTACCCTGAAATACAGGTCTACACTGTGGGTCCCGAATATGCACACGC
TGAAGCAAGAAAATCACCTGTTGTTGATGGTGTAGTAATGAGAAATCCAGATGGGAAGGA
AGTAAGTCTGCTGATCTTTACCAAACCATTCCACATATGAAATCTTATGTCGCTTTTTCC
ATGAACAATGGAGCTATGGTTAACAGGGTACTAAAGGCGGTCGAGTATATAGAGGAATTG
TAACTTATGTCTTATGATGGTATAGAATTTGATCAAAAATCAGTATTGTTATATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATGTCCGC
ACGCGTTATT
   
Sequence Name 02-K116517-0022-083-C06C-8409
GenBank Accession LN487192 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K116517-0022-083-C06C-8409
TTGAATATATAACAATACTGATTTTTGATCAAATTCTATACCATCATAAGACATAAGTTA
CAATTCCTCTATATACTCGACCGCCTTTAGTACCCTGTTAACCATAGCTCCATTGTTCAT
GGAAAAAGCGACATAAGATTTCATATGTGGAATGGTTTGGTAAAGATCAGCAGACTTACT
TCCTTCCCATCTGGATTTCTCATTACTACACCATCAACAACAGGTGATTTTCTTGCTTCA
GCGTGTGCATATTCGGGACCCACAGTGTAGACCTGTATTTCAGGGTAGACTGTCAGTCAA
AAACTCGTCAAAGCCAAGCTTCAACCAAAACATAATCCAGAAAAAAAAAACTATTCCAAA
TAGCCTTACGACATGGAAAAATATCCATTGCAGGAACAAACAGGACAACAAAACAGCATA
AGGACATTAACACGGCAACTATCTCAATTTATGAGACCTGGATAAACAAGAGCATAAAAA
CACATTCAATGTTTTAAAAAAGGTTAAGTAGCCTGGTCATGGATTAAAAC

Last Updated on 06.12.2018 16:11