Sequences from confirmation amplicon


Confirmation sequences for line 089D01 and hit At1g08765 are shown.
Line ID 089D01
Locus At1g08765 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:78881
Primers used for PCR 24by - 8474, show primer details
   
Sequence Name 62-K108477-0022-089-D01C-24by
GenBank Accession LN510776 [GenBank]
Sequencing primer gene specific primer, 24by: 5'-TTATACAATCTATTGGCCACGTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K108477-0022-089-D01C-24by
TTATTCTTTTTTTATATTTTTTAGCTCTAAACATAAATAGATTTTCAAAGTTTTTTATCG
TTTTTATTTTCAAATTCCTATAATCGTTTTTGTTTTATTTAGATTTATTTTATTTTTAAT
TATATTTTTATATACTACTATTAAAACGTATTATAAGGTTTTTTATAAGTGCTAAAATTT
TTAATTTTTTTAAACTAATCAGATACAATATCCAAAATACTAAAAAGTGTCTCTATCTTT
TTCTATTCAAAATATTGAGTTGTCTTTGACAATATTTTAAACTAGTTGTAAATCACAGAG
ATAAACACCACATAAATCAATAAATAACTGTAATCTTTACCTTGAAGATTTTATTCATAT
GAAATATCTTATAGAAAATACTTCGTTAGCTTGAAAATGCATATTTAGTTTAAATACAAA
TAAACGAAACAAAAAGCAAAAAGCATAGACGGATCCGTAAATCTACTCTTAAGAAGCCAT
GCAAGAAAGATTCAAAGTCATCACAAAAACCAAGCTTTTGTAATTTGCGCAAGTAATTAA
CAAAAAAAATGAAAGAGTGGTAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGTTATT
   
Sequence Name 46-K108476-0022-089-D01C-8409
GenBank Accession LN505885 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K108476-0022-089-D01C-8409
TCTTCATTTTTTTTGTTATACTTGCGCAAATTACAAAAGCTTGGTTTTTGTGATGACTTT
TAATCTTTCTTGCATGGCTTCTTAAGAGTAGATTTACGGATCCGTCTATGCTTTTTGCTT
TTTGTTTCGTTTATTTGTATTTAAACTAAATATGCATTTTCAAGCTAACGAAGTATTTTC
TATAAGATATTTCATATGAATAAAATCTTCAAGGTAAAGATTACAGTTATTTATTGATTT
ATGTGGTGTTTATCTCTGTGATTTACAACTAGTTTAAAATATTGTCAAAGACAACTCAAT
ATTTTGAATAGAAAAAGATAGAGACACTTTTTAGTATTTTGGATATTGTATCTGATTAGT
TTAAAAAAATTAAAAATTTTAGCACTTATAAAAAACCTTATAATACGTTTTAATAGTAGT
ATATAAAAATATAATTAAAAATAAAATAAATCTAAATAAAACAAAAACGATTATAGGAAT
TTGAAAATAAAAACGATAAAAAACTTTGAAAATCTATTTATGTTTAGAGCTAAAAAATAT
TAAAAAAAGAATAACTGAAAATATAGGATAGAAAATGACGTGGCCAATAGATTGTATAA
Primers used for PCR 02lg - 8474, show primer details
   
Sequence Name 32-K113916-0022-089-D01C-02lg
GenBank Accession LN500666 [GenBank]
Sequencing primer gene specific primer, 02lg: 5'-TTTTCTTACCGATTCGTGGATTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K113916-0022-089-D01C-02lg
TTTTTCTTGCCAGTTAACGTATGACGTGTCACCTCATTCGAAAATATAAGCACTAACCGC
GTCATTGATATTATAATAAATAAAATATATTTTTGAAAAAGCTAGGGTTTAGGAATGACG
ACCTGTTTCTGTTGTGTCTTATTAAAAGCCCATCTTCGTCTCCGCCACTCATCATTCCCT
CATCATAACACCATCATCACCATTCACCAACCTCTCTCTCTCTCTCCTCTATCACTCTCT
ACAACAAAAATTTGTGAATCTGCTAAGATCGATTATCATGAGGGTTAAGCTATTTCAGTT
GAGGGGAATGTTGTCTGGATCGAGGATATTATAGATATATACATGTGTATGTTAATGATT
CAAGTGATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCC
   
Sequence Name 04-K113915-0022-089-D01C-8409
GenBank Accession LN487999 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K113915-0022-089-D01C-8409
TACATTGAATCACTTGAATCATTAACATACACATGTATATATCTATAATATCCTCGATCC
AGACAACATTCCCCTCAACTGAAATAGCTTAACCCTCATGATAATCGATCTTAGCAGATT
CACAAATTTTTGTTGTAGAGAGTGATAGAGGAGAGAGAGAGAGAGGTTGGTGAATGGTGA
TGATGGTGTTATGATGAGGGAATGAGGAGTGGTGTGAGGGAAGATGGGC

Last Updated on 06.12.2018 16:11