Sequences from confirmation amplicon


Confirmation sequences for line 089F03 and hit At5g07410 are shown.
Line ID 089F03
Locus At5g07410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2345974
Primers used for PCR 61pm - 8474, show primer details
   
Sequence Name 73-K115263-0022-089-F03C-61pm
GenBank Accession LN512955 [GenBank]
Sequencing primer gene specific primer, 61pm: 5'-AGTTGTAGAAAGCAGCAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K115263-0022-089-F03C-61pm
TCCTTAGTTTTCCATCTGGTGCCGGTGCAGTGTTCTACATATACAAAAAAATAAATCACT
CGACCATGAAATAGTCGACTAGTCTCATAGATATGTATAATATATTAGTAGGATCACTTA
CCTTAACGACGATGTTAACGGCCATGAAATAGTCGGATAAGATAATGAGAGAGGCACTAT
CAACGGTTCCATACTTGGCGGCGGTACCGTCGTAGGTGATAACAGGCATGGCATTGGGTT
GTCCCATCAATGTAATGAAGGGTTTGTTCCTGTCGATAGTGACCTTCTCTCTGTACTCAC
CATGAGCCATCTTTATGATCACCCGCTTTGTGTTCCCTGCGGGAACGCTCTTTATTGCGT
CTGTTAGTGTTTTGAATTCACCTCCCTTTGGATTCACCTGCAATAATATTTACATGTTTT
CAAAAAATTTATGTTAAAATATATTGTTACAATGATGAGTTTGGGAAAATCAAGTGTGAG
TTATACGTTGATGATACGTGGAGCAGCCTCAGCAGCTACGAGAGCAGGATCTAAGCCTTT
ACGTTGAGCCAATGGACCAACGTGGGTATTGAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGGTTATT
   
Sequence Name 85-K115262-0022-089-F03C-8409
GenBank Accession LN514577 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K115262-0022-089-F03C-8409
TACCCACGTTGGTCCATTGGCTCAACGTAAAGGCTTAGATCCTGCTCTCGTAGCTGCTGA
GGCTGCTCCACGTATCATCAACGTATAACTCACACTTGATTTTCCCAAACTCATCATTGT
AACAATATATTTTAACATAAATTTTTTGAAAACATGTAAATATTATTGCAGGTGAATCCA
AAGGGAGGTGAATTCAAAACACTAACAGACGCAATAAAGAGCGTTCCCGCAGGGAACACA
AAGCGGGTGATCATAAAGATGGCTCATGGTGAGTACAGAGAGAAGGTCACTATCGACAGG
AACAAACCCTTCATTACATTGATGGGACAACCCAATGCCATGCCTGTTATCACCTACGAC
GGTACCGCCGCCAAGTATGGAACCGTTGATAGTGCCTCTCTCATTATCTTATCCGACTAT
TTCATGGCCGTTAACATCGTCGTTAAGGTAAGTGATCCTACTAATATATTATACATATCT
ATGAGACTAGTCGACTATTTCATGGTCGAGTGATTTATTTTTTTGTATATGTAGAACACT
GCACCGGCACCAGATGGTAAAACTAAGGGAGCACAAGCCTTATCCATGAGAATCTCCGGA
AACTTTGCTGCTTTCTACAACTA

Last Updated on 06.12.2018 16:11