Sequences from confirmation amplicon


Confirmation sequences for line 092H10 and hit At3g58480 are shown.
Line ID 092H10
Locus At3g58480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21629746
Primers used for PCR 04uj - 8474, show primer details
   
Sequence Name 51-K117452-0022-092-H10C-04uj
GenBank Accession LN507620 [GenBank]
Sequencing primer gene specific primer, 04uj: 5'-TAAGGAACTGCTTGAATCTGGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K117452-0022-092-H10C-04uj
ACACTTTCAGAACGTTTATTTGAAGGCATTTTGATCTGAGGCATATTTGTATTGATTGTG
CAGAGACAAAGACCATTCTTCGATCAATCAGTTTTAATGACATTGACTCGGATTCAACCA
TAACTGAATCACCTGAAGCTAGAAAGACGCTATACAACAGATCCCTTAGCGGTAAAGGGA
CACAACGATCAAGAATTTCTCCAGGAGATGTGGAGAACAATTTCTCTATTAAACCTTTAA
CTTTTATGAAAGAAGATGATAGATATAAAATGCGGATATGGAAACCTGTTTGCGAAAACG
AACATGCAAAAGAATTTTTAGCCTTGTTATTAGGTGATGGAACATATCAAGCTGCATTAA
AGTTACAGAAAGTTTATAGAAGTTTTCTGAACATATCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 28-K117451-0022-092-H10C-8409
GenBank Accession LN499022 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K117451-0022-092-H10C-8409
GAAAACTTCTATAAACTTTCTGTAACTTTAATGCAGCTTGATATGTTCCATCACCTAATA
ACAAGGCTAAAAATTCTTTTGCATGTTCGTTTTCGCAAACAGGTTTCCATATCCGCATTT
TATATCTATCATCTTCTTTCATAAAAGTTAAAGGTTTAATAGAGAAATTGTTCTCCACAT
CTCCTGGAGAAATTCTTGATCGTTGTGTCCCTTTACCGCTAAGGGATCTGTTGTATAGCG
TCTTTCTAGCTTCAGGTGATTCAGTTATGGTTGAATCCGAGTCAATGTCATTAAAACTGA
TTGATCGAAGAATGGTCTTTGTCTCTGCACAATCAATACAAATATGCCTCAGATCAAAAT
GCCTTCAAATAAACGTTCATAGAAGTTGTCACAAATACTAACCCATTTTCACTTGAAACC
AGAT

Last Updated on 06.12.2018 16:11