Sequences from confirmation amplicon


Confirmation sequences for line 094E09 and hit At5g44050 are shown.
Line ID 094E09
Locus At5g44050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17725866
Primers used for PCR BM10 - 8474, show primer details
   
Sequence Name 23-K015837-022-094-E09C-8409
GenBank Accession LN496343 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K015837-022-094-E09C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGATAGTAGTTTGAACA
TCAAATATGATGGCTAATCTTTGACACCAAACTTGAACATCCAGCCCATGACAATTCCAA
GTGGAAGTCCAATGAAATAGTAGCATCCCAAATTTATAAATGCGACTAGTGATTGCCAAC
CCGAACCAACCGCAACACCTAAGCAAAATCAAAAGTCACCACACAACCGTTGAACACGTT
TATATTCGGTTAACAAAGAAAAAAAATCCAAATCAATTTGTTTATGTAGCGTACCGGAGA
GAACCGGTTGGACACTGTTGAGAAGAATCGCAAAGGATAAAAGAATAGAGAGATTATTGA
CTGCTTTTAGGACAGTTTCACTTG
   
Sequence Name 24-K015837-022-094-E09C-BM10
GenBank Accession LN496782 [GenBank]
Sequencing primer gene specific primer, BM10: 5'-TCAAGTGAAACTGTCCTAAAAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K015837-022-094-E09C-bm10
ATAATCTCTCTATTCTTTTATCCTTTGCGATTCTTCTCAACAGTGTCCAACCGGTTCTCT
CCGGTACGCTACATAAACAAATTGATTTGGATTTTTTTTCTTTGTTAACCGAATATAAAC
GTGTTCAACGGTTGTGTGGTGACTTTTGATTTTGCTTAGGTGTTGCGGTTGGTTCGGGTT
GGCAATCACTAGTCGCATTTATAAATTTGGGATGCTACTATTTCATTGGACTTCCACTTG
GAATTGTCATGGGCTGGATGTTCAAGTTTGGTGTCAAAGATTAGCCATCATATTTGATGT
TCAAACTACTATCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11