Sequences from confirmation amplicon


Confirmation sequences for line 094F06 and hit At2g45590 are shown.
Line ID 094F06
Locus At2g45590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18787788
Primers used for PCR 06qz - 8474, show primer details
   
Sequence Name 63-K115869-0022-094-F06C-06qz
GenBank Accession LN511058 [GenBank]
Sequencing primer gene specific primer, 06qz: 5'-AAACCAAGAGTCAATGCTTGAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K115869-0022-094-F06C-06qz
CTCTTTTCCTCTTCTTTCAGTCAGCTCTTCACAAAACTCTTCCTTCCACCACTCTCTAGG
CTTTCTCTTCTTCTCTTTCTTCTTCTTCTTAGAGACTGATGATGACCCATCACCATTGTT
GATCCATTCCTTATTGTTGCTAGGTCTCTCTTTCTTAATCTCACTCCCAATCCATTCCAT
CACGTAATCCTTCACACTCCCTGATTCAATCCCTCCTCTGCTTCCACCATTGTTGTCCTG
TTTCCACCACCAATCTCTCTTCGATCCCACTTTAAGCTTGCTACTTTCCGGTGACAATCC
AGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCACAAATTA
   
Sequence Name 22-K115867-0022-094-F06C-8409
GenBank Accession LN496257 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K115867-0022-094-F06C-8409
ATTGAATATATCCTGGATTGTCACCGGAAAGTAGCAAGCTTAAAGTGGGATCGAAGAGAG
ATTGGTGGTGGAAACAGGACAACAATGGTGGAAGCAGAGGAGGGATTGAATCAGGGAGTG
TGAAGGATTACGTGATGGAATGGATTGGGAGTGAGATTAAGAAAGAGAGACCTAGCAACA
ATAAGGAATGGATCAACAATGGTGATGGGTCATCATCAGTCTCTAAGAAGAAGAAGAAAG
AGAAGAAGAGAAAGCCTAGAGAGTGGTGGAAGGAAGAGTTTTGTGAAGAGCTGACTAGAA
AGAAGAGGAAAAAGAAGAAGAAGAAGAAAAGAGGGTTAAGCTCAATCTCAAGCATTGACT
CTTGGTT

Last Updated on 06.12.2018 16:11