Sequences from confirmation amplicon


Confirmation sequences for line 097D04 and hit At5g57220 are shown.
Line ID 097D04
Locus At5g57220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23188993
Primers used for PCR MR23 - 8474, show primer details
   
Sequence Name 33-K015724-022-097-D04C-8409
GenBank Accession LN500782 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K015724-022-097-D04C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATAAAAACATATGTTTTTAT
AAAGATATTATATGTGTACATGTGTAGATGAACTAACAACTTGTGTGTGAATTTAATGTA
TATAGAGTATGATGCTAGCTGGAACGGATACTGCGGCCGTAACATTGGAATGGGCGATGG
CAAATTTATTAAAAAAACCTGAAGTGTTGAAAAAGGCAAAAGCTGAGATAGACGAGAAGA
TTGGAGAAGAGAG
   
Sequence Name 34-K015724-022-097-D04C-MR23
GenBank Accession LN501205 [GenBank]
Sequencing primer gene specific primer, MR23: 5'-TCTCTCTTCTCCAATCTTCTCGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K015724-022-097-D04C-MR23
GCTTTGCCTTTTTCAACACTTCAGGTTTTTTTAATAAATTTGCCATCGCCCATTCCAATG
TTACGGCCGCAGTATCCGTTCCAGCTAGCATCATACTCTATATACATTAAATTCACACAC
AAGTTGTTAGTTCATCTACACATGTACACATATAATATCTTTATAAAAACATATGTTTTT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCC
GTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGA
CGTGTCTACATTCACGTCCAAATGGGGGCTTAGATGAGAAACTTCACGATCGATGCCTTG
ATTTCGCCATTCCCAGATACCCATTTCATCTTCAGATTGGTCTGAGATTATGCGAAAATA
TACACTCATATACATAAATACTGACAGTTTGAGCTACCAATTCAGGGTAGCC
Primers used for PCR 09lq - 8474, show primer details
   
Sequence Name 75-K118152-0022-097-D04C-09lq
GenBank Accession LN513300 [GenBank]
Sequencing primer gene specific primer, 09lq: 5'-GTCCGTTACCAAACACCATTAGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K118152-0022-097-D04C-09lq
CGCTCAGCATAACTTCTCGGGCTCATCCCAAAGTCTTGGGTCTCTATGGATGGCCCAAGC
GTTCACTAGCACGATAGTGCCACGTGGTATGTCGTATCCACCAATCTTGAGATCTTCAGA
TGGAGAACGTGGAACAAGAAGTGGTGCAGCTGGACACAACCGAAAAGTCTCGGAAACTAT
GTTTTGGAGATAAGGTAGATTCGCAATGTCAGGTTCATCGACCAATCTCTCTTCTCCAAT
CTTCTCGTCTATCTCAGCTTTTGCCTTTTTCAACACTTCAGGTTTTTTTAATAAATTTGC
CATCGCCCATTCCAATGTTACGGCCGCAGTATCCGTTCCAGCTAGCATCATACTCTATAT
ACATTAAATTCACACACAAGTTGTTAGTTCATCTACACATGTACACATATAATATCTTTA
TAAAAACATATGTTTTTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCACGGTTTATTAT
   
Sequence Name 27-K118151-0022-097-D04C-8409
GenBank Accession LN498590 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K118151-0022-097-D04C-8409
GCCATTTCATTGAATATAAAACATATGTTTTTATAAAGATATTATATGTGTACATGTGTA
GATGAACTAACAACTTGTGTGTGAATTTAATGTATATAGAGTATGATGCTAGCTGGAACG
GATACTGCGGCCGTAACATTGGAATGGGCGATGGCAAATTTATTAAAAAAACCTGAAGTG
TTGAAAAAGGCAAAAGCTGAGATAGACGAGAAGATTGGAGAAGAGAGATTGGTCGATGAA
CCTGACATTGCGAATCTACCTTATCTCCAAAACATAGTTTCCGAGACTTTTCGGTTGTGT
CCAGCTGCACCACTTCTTGTTCCACGTTCTCCATCTGAAGATCTCAAGATTGGTGGATAC
GACATACCACGTGGCACTATCGTGCTAGTGAACGCTTGGGCCATCCATAGAGACCCAAGA
CTTTGGGATGAGCCCGAGAAGTTTATGCCTGAGCGGTTCGAAGACCAAGAAGCTTCAAAG
AAACTAATGGTGTTTGGTAACGGA
Primers used for PCR 72wh - 8474, show primer details
   
Sequence Name 45-K118301-0022-097-D04C-72wh
GenBank Accession LN505679 [GenBank]
Sequencing primer gene specific primer, 72wh: 5'-ACGATTACACCACTATAGGAACCG-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K118301-0022-097-D04C-72wh
TCTCCGCCGTATTTGCTCTCTGAAATCCTCTCCTCTAACCGTCTCACCGGATTCCTCTCC
GTACGTAAAGACGAGATTCGTCGGCTGCTCACAAAACTCTCACGTGAATATGACGGCCGT
GTTGTCGAGCTTGAACCTCTTCTTGCAGATTTGACGTTCAATAATATTGTCCGCATGGTC
ACAGGGAGACGCTACTACGGAGACCAGGTTCGTTATATAATTAATTCGCTTAATATCAAC
ATTTTCACTAACCTTTATACACTTTCTACCATTCTTGTAATTAAAAGTTTTTACTAACCT
TATTAAATATTTATTCCTTAAAGGTTCATAACAAGGAAGAAGCGAATCTATTCAAGAAAC
TAGTGACGGACATCAATGACAACAGTGGTGCGAGCCATCCAGGAGATTATCTACCGATTC
TTAAAGTGTTCGGACACGGCTACGAGAAAAAAGTGAAAGCACTAGGCGAAGCCATGGACG
CTTTCTTACAACGACTGCTCGATGAATGTCGAATAAATGGAGAGAGCAACACAATGGTTA
GTCACTTGTTGTCTTTACAACTAGACCAACCCAAGTATTACAGTGACGTCATCATCAAAG
GGCTCATGCTCGTAAGTATTCTCATACATATAAACCTTAAATATAATTTTTTTTCCTTGG
ACTCACTAAATGCAAACATAAAACTGAAAGAAGAAAGATGGTTTATGTTGTCAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAG
   
Sequence Name 13-K118300-0022-097-D04C-8409
GenBank Accession LN492251 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K118300-0022-097-D04C-8409
CTTTACATTGAATATATCCTGACACATAAACCATCTTTCTTCTTTCAGTTTTATGTTTGC
ATTTAGTGAGTCCAAGGAAAAAAAATTATATTTAAGGTTTATATGTATGAGAATACTTAC
GAGCATGAGCCCTTTGATGATGACGTCACTGTAATACTTGGGTTGGTCTAGTTGTAAAGA
CAACAAGTGACTAACCATTGTGTTGCTCTCTCCATTTATTCGACATTCATCGAGCAGTCG
TTGTAAGAAAGCGTCCATGGCTTCGCCTAGTGCTTTCACTTTTTTCTCGTAGCCGTGTCC
GAACACTTTAAGAATCGGTAGATAATCTCCTGGATGGCTCGCACCACTGTTGTCATTGAT
GTCCGTCACTAGTTTCTTGAATAGATTCGCTTCTTCCTTGTTATGAACCTTTAAGGAATA
AATATTTAATAAGGTTAGTAAAAACTTTTAATTACAAGAATGGTAGAAAGTGTATAAAGG
TTAGTGAAAATGTTGATATTAAGCGAATTAATTATATAACGAACCTGGTCTCCGTAGTAG
CGTCTCCCTGTGACCATGCGGACAATATTATTGAACGTCAAATCTGCAAGAAGAGGTTCA
AGCTCGACAACACGGCCGTCATATTCACGTGAGAGTTTTGTGAGCAGCCGACGAATCTCG
TCTTTACGTACGGAGAGGAATCCGGTGAGACGGTTAGAGGAGAGGATTTCTAGAGAGCAA
ATACGGCGGAGATTACGCCAATGGTCACCGTAAGCGGCGGTTC

Last Updated on 06.12.2018 16:11