Sequences from confirmation amplicon


Confirmation sequences for line 098F10 and hit At1g01040 are shown.
Line ID 098F10
Locus At1g01040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30997
Primers used for PCR KS26 - 8474, show primer details
   
Sequence Name 54-K031690-022-098-F10C-8409
GenBank Accession LN508375 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K031690-022-098-F10C-8409
CATTTACAATTGAATATATCCTGGCCGTCCTGGTGCGAACCAATGCCGAGTGTTAAGAAA
GCTAAGGATTCAGCTGCGGTTCTTCTACTTGAGCTTTTAAATAAAACTTTTTCTTGATTC
TTTTACTCTCTTCAACGAGATGTAGTCATTACATTTTAAACCTTAAAACCATAGTGGTTG
TAGTGTTTTAATTGATCTGTTGTTATTCGAGTTTAATTGGTTTATAGAACTCTTCAAACA
AATTAAACCAAAAATTTCAATGCCAAGAAAGGGTCTTTAAAACGAAATTACAGAAGGACC
AAATGATAAGGAAGAAAAATGCAGAGATAAAAGTAATATCAATTAGGATCATATGCTTCT
TATTATCAATGAAAAGTAACAGAAACATAGATGCTGCAGAAATCTTCTGAGGAGAAGCTT
CAACGCCTCAGGGTGTGGAGAATGTATTCAGCATAGAGGTCCCTGTAATGGGGAATTCAG
AATCCAGAGTTGCTTTAATGTTACAAACTAAAAAAGATGTAAGAGAGTTTGGTTCTTGAT
AAAGAAACAGAGGCTTACATTGAGTACTGGATAGCTTCAACCGCAGACTCAGATGGCAAA
AAATCA
   
Sequence Name 06-K031690-022-098-F10C-KS26
GenBank Accession LN488689 [GenBank]
Sequencing primer gene specific primer, KS26: 5'-AATGATTTTCTGCCATCTGAGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K031690-022-098-F10C-KS26
TAGCCTCTGTTTCTTTATCAAGAACAAACTCTCTTACATCTTTTTTAGTTTGTAACATTA
AAGCAACTCTGGATTCTGAATTCCCCATTACAGGGACCTCTATGCTGAATACATTCTCCA
CACCCTGAGGCGTTGAAGCTTCTCCTCAGAAGATTTCTGCAGCATCTATGTTTCTGTTAC
TTTTCATTGATAATAAGAAGCATATGATCCTAATTGATATTACTTTTATCTCTGCATTTT
TCTTCCTTATCATTTGGTCCTTCTGTAATTTCGTTTTAAAGACCCTTTCTTGGCATTGAA
ATTTTTGGTTTAATTTGTTTGAAGAGTTCTATAAACCAATTAAACTCGAATAACAACAGA
TCAATTAAAACACTACAACCACTATGGTTTTAAGGTTTAAAATGTAATGACTACATCTCG
TTGAAGAGAGTAAAAGAATCAAGAAAAAGTTTTATTTAAAAGCTCAAGTAGAAGAACCGC
AGCTGAATCCTTAGCTTTCTTAACACTCGGCATTGGTTCGCACCAGGACGGCCAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACG
TGTCTACAT

Last Updated on 06.12.2018 16:11