Sequences from confirmation amplicon


Confirmation sequences for line 100H01 and hit At1g01190 are shown.
Line ID 100H01
Locus At1g01190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:84299
Primers used for PCR MR24 - 8474, show primer details
   
Sequence Name 35-K015725-022-100-H01C-8409
GenBank Accession LN501616 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K015725-022-100-H01C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGATGGGTTTGGTTTTC
GGGAGAGAGTATGAATTGGAGTCAAATAACAACTTGGAATCTGAATGCTTAAAGGGTTTG
GTTGAAGAAGGGTACGATCTTCTAGGTACGTTAAATTGGACCGACCATCTTCCTTGGTTA
GCCGGTTTAGATTTCCAACAAATCCGGTTTAGGTGCTCGCAGCTCGTACCGAAAGTAAAT
CTGTTATTGAGCCGTATCATACATGAAC
   
Sequence Name 36-K015725-022-100-H01C-MR24
GenBank Accession LN502023 [GenBank]
Sequencing primer gene specific primer, MR24: 5'-TGTTCATGTATGATACGGCTCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K015725-022-100-H01C-MR24
AGATTTACTTTCGGTACGAGCTGCGAGCACCTAAACCGGATTTGTTGGAAATCTAAACCG
GCTAACCAAGGAAGATGGTCGGTCCAATTTAACGTACCTAGAAGATCGTACCCTTCTTCA
ACCAAACCCTTTAAGCATTCAGATTCCAAGTTGTTATTTGACTCCAATTCATACTCTCTC
CCGAAAACCAAACCCATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTC
TAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGGGGGCTTAGATGA
GAAACTTCACGATCGATGCCTTGATTTCGCCATTCCCAGATACCCATTTCATCTTCAGAT
TGGTCTGAGATTATGCGAAAATATACACTCATATACATAAATACTGACAGTTTGAGCTAC
CAATT
Primers used for PCR 73wh - 8474, show primer details
   
Sequence Name 48-K118301-0022-100-H01C-73wh
GenBank Accession LN506636 [GenBank]
Sequencing primer gene specific primer, 73wh: 5'-TTGGCTAAGACCTTCATAGTTCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K118301-0022-100-H01C-73wh
AACATAAATCACAACCTATAAACGGAAAAACAAAATCACAAACAAAAAAAGAAGAAGAAG
AACATTTATCCTATCCATAATGTTCTCTTTGAACATGAGAACCGAAATAGAAAGTTTGTG
GGTATTTGCTCTTGCATCAAAATTCAATATTTACATGCAACAACATTTTGCTTCCCTTCT
CGTCGCCATTGCTATCACTTGGTTTACCATAACCATCGTATTTTGGTCTACTCCGGGTGG
ACCGGCTTGGGGAAAATACTTCTTCACTCGCCGGTTTATTTCTCTCGATTACAACCGAAA
ATACAAGAATCTCATTCCCGGTCCTAGAGGGTTTCCACTTGTGGGAAGCATGAGCCTTAG
GTCAAGCCACGTGGCTCATCAGCGCATAGCGTCTGTGGCTGAGATGAGTAACGCCAAGCG
GCTCATGGCGTTTAGCCTCGGTGATACTAAGGTGGTGGTGACGTGTCATCCTGCCGTGGC
AAAGGAGATACTAAACAGTTCGGTTTTTGCTGACCGACCGGTTGACGAAACCGCTTACGG
TTTGATGTTTAACCGAGCCATGGGATTTGCTCCCAATGGTACTTATTGGCGTACGCTGCG
TCGGTTAGGCTCGAACCATCTTTTTAACCCGAAGCAAATCAAACAATCGGAGGATCAGAG
ACGGGTGATAGCGACTCAGATGGTGAATGCGTTTGCAGTGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACCG
   
Sequence Name 16-K118300-0022-100-H01C-8409
GenBank Accession LN493626 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K118300-0022-100-H01C-8409
CTCTGATCCTCCGATTGTTTGATTTGCTTCGGGTTAAAAAGATGGTTCGAGCCTAACCGA
CGCAGCGTACGCCAATAAGTACCATTGGGAGCAAATCCCATGGCTCGGTTAAACATCAAA
CCGTAAGCGGTTTCGTCAACCGGTCGGTCAGCAAAAACCGAACTGTTTAGTATCTCCTTT
GCCACGGCAGGATGACACGTCACCACCACCTTAGTATCACCGAGGCTAAACGCCATGAGC
CGCTTGGCGTTACTCATCTCAGCCACAGACGCTATGCGCTGATGAGCCACGTGGCTTGAC
CTAAGGCTCATGCTTCCCACAAGTGGAAACCCTCTAGGACCGGGAATGAGATTCTTGTAT
TTTCGGTTGTAATCGAGAGAAATAAACCGGCGAGTGAAGAAGTATTTTCCCCAAGCCGGT
CCACCCGGAGTAGACCAAAATACGATGGTTATGGTAAACCAAGTGATAGCAATGGCGACG
AGAAGGGAAGCAAAATGTTGTTGCATGTAAATATTGAATTTTGATGCAAGAGCAAATACC
CACAAACTTTCTATTTCGGTTCTCATGTTCAAAGAGAACATTATGGATAGGATAAATGTT
CTTCTTCTTCTTTTTTTGTTTGTGATTTTGTTTTTCCGTTTATAGGTTGTGATTTATGTT
TGGGTCTTTGTGAGGCTTTAAATAGGCGTACGAACTATG

Last Updated on 06.12.2018 16:11