Sequences from confirmation amplicon


Confirmation sequences for line 102C09 and hit At1g78820 are shown.
Line ID 102C09
Locus At1g78820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29635629
Primers used for PCR UU03 - 8474, show primer details
   
Sequence Name 01-K017253-022-102-C09C-UU03
GenBank Accession LN486370 [GenBank]
Sequencing primer gene specific primer, UU03: 5'-ATCAGATGTACGGCTAACGAGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K017253-022-102-C09C-UU03
GGATTGGCCGACGAGGAGTGTATCGGTGGGATGATCGAAACTCTGCCAAACGAATTTTCC
ATGTTTGTCGTGTAGCACCATGTTTCCATTGGGCAAGATTTGGAATCCGGTGACGCCTTT
GTTGGCTGTGTTAGTCTGCCACTTGACTTGGCCGTTTAACTCAGCGAGCACAAGGTTTCC
GTTTCGGCCGAATGAGAGTGTGGAGTTGTCTCCGACGGGGTTGTTGCGATTGGCGTCCCA
GATCCAACGCGTGAAACTCATGTCACGCCTCGTTCCGACTCGGAGAGCTAACACGTAGGC
GCTAGGAGTTGTATTGTAAAACATGAGCTGGAAAGGAATTGTGAAGAAATTCTGATTCGG
TGAGTTGAGGAATCGGTAACTCGCGTCGTACTCGGTGATGTATGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCG
TATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG
TCTACATTCAC
   
Sequence Name 02-K017253-022-102-C09C-8409
GenBank Accession LN486820 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K017253-022-102-C09C-8409
CGAGTTACCGATTCCTCAACTCACCGAATCAGAATTTCTTCACAATTCCTTTCCAGCTCA
TGTTTTACAATACAACTCCTAGCGCCTACGTGTTAGCTCTCCGAGTCGGAACGAGGCGTG
ACATGAGTTTCACGCGTTGGATCTGGGACGCCAATCGCAACAACCCCGTCGGAGACAACT
CCACACTCTCATTCGGCCGAAACGGAAACCTTGTGCTCGCTGAGTTAAACGGCCAAGTCA
AGTGGCAGACTAACACAGCCAACAAAGGCGTCACCGGATTCCAAATCTTGCCCAATGGAA
ACATGGTGCTACACGACAAACATGGAAAATTCGTTTGGCAGAGTTTCGATCATCCCACCG
ATACACTCCTCGTCGGCCAATCCCTAAAAGTTAACGGAGTTAACAAACTCGTTAGCCGTA
CATCTGA

Last Updated on 06.12.2018 16:11