Sequences from confirmation amplicon


Confirmation sequences for line 102C12 and hit At3g18860 are shown.
Line ID 102C12
Locus At3g18860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6503793
Primers used for PCR AK02 - 8474, show primer details
   
Sequence Name 23-K016844-022-102-C12C-8409
GenBank Accession LN496347 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K016844-022-102-C12C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAAAAATAAGAGAAGAC
TCAAGTATCATCTAACAGTTCAATCAGGTTATGGGCACTAAGTGGCGAAGTTCTTCTTGA
GATGGTTGGCCACACCTCCCTTGTTTATTCAGTTGACGCACATTCGTCTGGCCTTATTGT
TAGTGCCAGTGAAGATCGTCATGCCAAGATATGGAAAGGTGATTACGGGTTCCTTAGTTT
CTATTGATGCCTTGGCTGTTCTTCACTTATGTTTCACAGTTTTCTAGGTTATATGAGGTC
TGAGATGTTTAAATTTGGAAAATATGTCTCCACTTTTCAGTATGTTGCTCGCTCTTAAAG
TTTTCTTTCAATAATAAAGATGTCTATATATCATATTTTTGTGGAAGACGTGTTATAAAT
TTGATCACTTAGTTGCAACCTATGGAAAGTTAGTTGTTAGTATGTCAGACAGTAATCAAC
TGATCAGAAA
   
Sequence Name 24-K016844-022-102-C12C-AK02
GenBank Accession LN496789 [GenBank]
Sequencing primer gene specific primer, AK02: 5'-TTCAAACTGGTTTCTGATCAGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K016844-022-102-C12C-ak02
TTACTGTCTGACATACTAACAACTAACTTTCCATAGGTTGCAACTAAGTGATCAAATTTA
TAACACGTCTTCCACAAAAATATGATATATAGACATCTTTATTATTGAAAGAAAACTTTA
AGAGCGAGCAACATACTGAAAAGTGGAGACATATTTTCCAAATTTAAACATCTCAGACCT
CATATAACCTAGAAAACTGTGAAACATAAGTGAAGAACAGCCAAGGCATCAATAGAAACT
AAGGAACCCGTAATCACCTTTCCATATCTTGGCATGACGATCTTCACTGGCACTAACAAT
AAGGCCAGACGAATGTGCGTCAACTGAATAAACAAGGGAGGTGTGGCCAACCATCTCAAG
AAGAACTTCGCCACTTAGTGCCCATAACCTGATTGAACTGTTAGATGATACTTGAGTCTT
CTCTTATTTTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAA
ACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTC
GGAAATCTTTATTTCGACGTGTCTACATTCACGC

Last Updated on 06.12.2018 16:11