Sequences from confirmation amplicon


Confirmation sequences for line 102G11 and hit At1g01180 are shown.
Line ID 102G11
Locus At1g01180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:75926
Primers used for PCR FT17 - 3144, show primer details
   
Sequence Name 52-K100897-0022-102-G11C-3144
GenBank Accession LN507748 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K100897-0022-102-G11C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAT
CGATTTCCCACCACCGCACGCCAAGAAGTTTCTCCGGCCAAAAAGAATCAAAGGTTGGGG
ATCATACGGGGCCGTTTTCGAGAATCTAATCCGCCGAGTCAAACCTAAGACGATCGTCGA
AGTCGGGAGTTTTCTCGGCGCGTCGGCGATACACATGGCGAATCTCACGCGCCGACTTGG
GCTCGAGGAGACTCAGATTCTATGCGTCGACGACTTCCGTGGCTGGCCCGGTTTTCGAGA
CCGGTTTAAGGACATGGCTATGGGTTAA
   
Sequence Name 52-K100905-0022-102-G11C-FT17
GenBank Accession LN507749 [GenBank]
Sequencing primer gene specific primer, FT17: 5'-GTTAACCAAAGCCATGTCCTTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K100905-0022-102-G11C-FT17
CGGGCGCCCGGAAGTCGTCGACGCATAGAATCTGAGTCTCCTCGAGCCCAAGTCGGCGCG
TGAGATTCGCCATGTGTATCGCCGACGCGCCGAGAAAACTCCCGACTTCGACGATCGTCT
TAGGTTTGACTCGGCGGATTAGATTCTCGAAAACGGCCCCGTATGATCCCCAACCTTTGA
TTCTTTTTGGCCGGAGAAACTTCTTGGCGTGCGGTGGTGGGAAATCGATCAAACACTGAT
AGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAAT
GAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCAT
CCCTTACGTCAGTGGAGATAT
Primers used for PCR FT16 - 8474, show primer details
   
Sequence Name 51-K100897-0022-102-G11C-8409
GenBank Accession LN507434 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K100897-0022-102-G11C-8409
GCCATTTACAATTGAATATTTCCCGGAGAAGTTCCGTTAAAGATCCGGTCAATAACAGTC
TGGCGAATCAATTCCGGCGGAACAAGATTGCCGCATGGAGTCGTAACTCGGAAGTTGTCG
ATTGAACAAACTTGAGGTTTTGTCGTTTCCACGGCTGTCGTAGACGGTGGCAGCTGCTGC
TGCAGCGGTTGATGATAGTGGTAGGCGGAGAAGTAACCAAAGGCGTAGGTTAGAACTATG
ACGATAACGTACGCAGCCTTGGTTTTCAAGAATTGGAAGGCGTCGCGTTTGTGAGGAAGA
CGACGATCCTCCTTCATGGTCAAGATTTCTGAGAATGAAGAATCAAGTAAAAACAAAATA
GGATTAAGGAAGGATAATTGTTTTTGTTATTAAGTTTATATAATTCGAATTATGGAAATT
TATTTATGATTATNTTTGGCGGG
   
Sequence Name 51-K100905-0022-102-G11C-FT16
GenBank Accession LN507435 [GenBank]
Sequencing primer gene specific primer, FT16: 5'-CCCGCCAAAATAATCATAAATAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K100905-0022-102-G11C-FT16
ATACTTAATAACAAAACATTATCCTTCCTTAATCCTATTTTGTTTTTACTTGATTCTTCA
TTCTCAGAAATCTTGACCATGAAGGAGGATCGTCGTCTTCCTCACAAACGCGACGCCTTC
CAATTCTTGAAAACCAAGGCTGCGTACGTTATCGTCATAGTTCTAACCTACGCCTTTGGT
TACTTCTCCGCCTACCACTATCATCAACCGCTGCAGCAGCAGCTGCCACCGTCTACGACA
GCCGTGGAAACGACAAAACCTCAAGTTTGTTCAATCGACAACTTCCGAGTTACGACTCCA
TGCGGCAATCTTGTTCCGCCGGAATTGATTCGCCAGACTGTTATTGACCGGATCTTTAAC
GGAACTTCTCCGGGAAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11