Sequences from confirmation amplicon


Confirmation sequences for line 105E07 and hit At5g07120 are shown.
Line ID 105E07
Locus At5g07120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2208507
Primers used for PCR TG01 - 8474, show primer details
   
Sequence Name 07-K023372-022-105-E07C-8409
GenBank Accession LN489081 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K023372-022-105-E07C-8409
ATTTCCCGGACATGAAGCCATTTACCCGGCTGGACACAGCACCATCCAACATCCTAGAAG
CCACATCTGTGCTCGTAGCAAGCGGTAACTTCCCTTGCGCTTGAAGAAAAACCTTCAATT
CATCACTGTTCCTGATCACAGGATGTGCAACAAGCCTACGCAAATACTTCTCCAATGCAA
CTCTTCTCTGCTCCACAAACTCTTGCTTTTGCATCACTTGACTCTCAACTATACTCTTAT
CTGGCCTCGGTGGAATGCAGAAACCTCTATAAGACTCAGCTAATCTATCAGCCAATGTAA
CAATGTCTCTGAATCTTCTCCTCACGCTAAACTCCGATCCACCGTAGTCAGAGAGATTCG
TTCTAGTTGTAATCTGGTAAGTGATGTAAGTACTTCCTCCAGGAATCATTGAATTTGTGG
CCTCTTGCTCTTTCTGAGGGTTAGAAACAGTGATCTTGATGTAATCAGAGCTCAAAA
   
Sequence Name 08-K023372-022-105-E07C-TG01
GenBank Accession LN489547 [GenBank]
Sequencing primer gene specific primer, TG01: 5'-CGTCTTCTTTGAGCTCTGATTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K023372-022-105-E07C-TG01
GATCACTGGTTTCTAACCCTCAGAAAGAGCAAGAGGCCACAAATTCAATGATTCCTGGAG
GAAGTACTTACATCACTTACCAGATTACAACTAGAACGAATCTCTCTGACTACGGTGGAT
CGGAGTTTAGCGTGAGGAGAAGATTCAGAGACATTGTTACATTGGCTGATAGATTAGCTG
AGTCTTATAGAGGTTTCTGCATTCCACCGAGGCCAGATAAGAGTATAGTTGAGAGTCAAG
TGATGCAAAAGCAAGAGTTTGTGGAGCAGAGAAGAGTTGCATTGGAGAAGTATTTGCGTA
GGCTTGTTGCACATCCTGTGATCAGGAACAGTGATGAATTGAAGGTTTTTCTTCAAGCGC
AAGGGAAGTTACCGCTTGCTACGAGCACAGATGTGGCTTCTAGGATGTTGGATGGTGCTG
TGTCCAGCCGGGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTACATT

Last Updated on 06.12.2018 16:11