Sequences from confirmation amplicon


Confirmation sequences for line 108H10 and hit At3g59570 are shown.
Line ID 108H10
Locus At3g59570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22001881
Primers used for PCR PC43 - 8474, show primer details
   
Sequence Name 60-K103762-0022-108-H10C-PC43
GenBank Accession LN510241 [GenBank]
Sequencing primer gene specific primer, PC43: 5'-ATTTAATTACTATTGCACCCGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K103762-0022-108-H10C-PC43
TCTCGCAACGCACCCATTATCAAACGCTCAACCAGTCGTTGAAAACGCTTGATATCGTTT
GAAACCGCAACCGTCCGTTTCCGCAAACTCCCGCAACCGCAGCCGCCGCGTTTAAACCAG
TCAGGCCCTTACTAGAATTTGTGTCTCACTCATGATAACAAATGGATTTTAGTTATGTAG
TGGTTGCAAGAGACTGGTAGAAAAGAGAATCTTGGATTTCTTATTATACATATGTGCCTT
CACGTACCAGATTCAAAACTCTTGCATATGTATTCATGGATCTTTATCTGTTGTAGATGA
TGTGGGCTGCTGATTTTGATGAGTCTTTTGCTGAAACTTTAGAGAAGGATTGCTTGAAGC
CGTTGGTTGTTCAGCTTCCAAAACGATCTGGAGTTGATATGGGGGACCATAAAATAGATG
ATGGAAATGGCACAACTACAAATAGTGAATCAACTTCAAAGAGCGACCGGACTTCAAAGA
GCAGCAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 52-K103763-0022-108-H10C-8409
GenBank Accession LN507793 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K103763-0022-108-H10C-8409
GTCGCTCTTTGAGTTGATTCACTATTTGTAGTTGTGCCATTTCCATCATCTATTTTATGG
TCCCCCATATCAACTCCAGATCGTTTTGGAAGCTGAACAACCAACGGCTTCAAGCAATCC
TTCTCTAAAGTTTCAGCAAAAGACTCATCAAAATCAGCAGCCCACATCATCTACAACAGA
TAAAGATCCATGAATACATATGCAAGAGTTTTGAATCTGGTACGTGAAGGCACATATGTA
TAATAAGAAATCCAAGATTCTCTTTTCTACCAGTCTCTTGCAACCACTACATAACTAAAA
TCCATTTGTTATCATGAGTGAGACACAAATTCTAGTAAGGGCCTGACTGGTTTAAACGCG
GCGGCTGCGGTTGCGGGAGTTTGCGGAAACGGACGGTTGCGGTTTCAAACGATATCAAGC
GTTTTCAACGACTGGTTGAGCGTTTGATAATGGGTGCGTTTGCGAGAGTTTTATGACTGG
TAAACCAGCGGGTGCAATAGAAATTAAA

Last Updated on 06.12.2018 16:11