Sequences from confirmation amplicon


Confirmation sequences for line 109D06 and hit At3g54320 are shown.
Line ID 109D06
Locus At3g54320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20117303
Primers used for PCR KR10 - 8474, show primer details
   
Sequence Name 14-K030599-022-109-D06C-8409
GenBank Accession LN492384 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K030599-022-109-D06C-8409
TAGTGCTTAGAGTTGACACTAGGTTGGTGTTTTAATTATTGTTAATTAACTCAGTAATCG
TACGTTCGTGTATTAATTATATACACAAATTGTTGCGATGACTTAATTAAGTCACAAGTT
TGGACTCTTTAGTGTTTAAAGCGGCG
   
Sequence Name 53-K030599-022-109-D06C-KR10
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, KR10: 5'-TACAAAGACCATTTCTCCTCCACT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K030599-022-109-D06C-KR10
TTTCAATTTCAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATTTCGACGTGTCT

Last Updated on 06.12.2018 16:11