Sequences from confirmation amplicon


Confirmation sequences for line 109F02 and hit At1g73600 are shown.
Line ID 109F02
Locus At1g73600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27671613
Primers used for PCR MS10 - 8474, show primer details
   
Sequence Name 19-K016386-022-109-F02C-8409
GenBank Accession LN494544 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K016386-022-109-F02C-8409
GATTTCCCGGACATGAAGCCATTTACAATTGACCAAATATGAACTTTCCAAATGAGTCTA
TGGATCTGATATTCTCCAACTGGCTGCTAATGTATCTCTCTGATCAAGAGGTAGCCATAT
TATTTATTACTGAGGGAACCACTTTTAAACTGTACACACTTAGACTAATCGCTTATATCC
CTTGGACAAATTTTCATGTCTTTTCATCAAATTGCAGGTTGAAGATTTGGCGAAAAAGAT
GTTACAATGGACAAAGGTTGGCGGGTATATTTTCTTTCGGGAGTCTTGTTTCCATCAGTC
TGGTGATAACAAGCGGAAGTACAACCCAACACACTACCGTGAACCTAAATTTTACACAAA
GGTGTGCTACCTATAAATCCCTCCACTCTTCTGTTTCTAGACGACAATTATTTGAACTCT
GGTACATGAATTCCTCTCAGCTTTTCAAAGAATGCCATATGAATGACGAAGATGGGAATT
CGTATGAACTCTCTTTGGTTAGCTGTAAATGCATTGGAGCTTATG
   
Sequence Name 20-K016386-022-109-F02C-MS10
GenBank Accession LN494994 [GenBank]
Sequencing primer gene specific primer, MS10: 5'-TTGTTTCTCACATAAGCTCCAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K016386-022-109-F02C-ms10
CATTTCAGCTAACCAAAGAGAGTTCATACGAATTCCCATCTTCGTCATTCATATGGCATT
CTTTGAAAAGCTGAGAGGAATTCATGTACCAGAGTTCAAATAATTGTCGTCTAGAAACAG
AAGAGTGGAGGGATTTATAGGTAGCACACCTTTGTGTAAAATTTAGGTTCACGGTAGTGT
GTTGGGTTGTACTTCCGCTTGTTATCACCAGACTGATGGAAACAAGACTCCCGAAAGAAA
ATATACCCGCCAACCTTTGTCCATTGTAACATCTTTTTCGCCAAATCTTCAACCTGCAAT
TTGATGAAAAGACATGAAAATTTGTCCAAGGGATATAAGCGATTAGTCTAAGTGTGTACA
GTTTAAAAGTGGTTCCCTCAGTAATAAATAATATGGCTACCTCTTGATCAGAGAGATACA
TTAGCAGCCAGTTGGAGAATATCAGATCCATAGACTCATTTGGAAAGTTCATATTTGGTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAAAAGAGTAATTACCCATTTTTTTTCAATTTCAAAA

Last Updated on 06.12.2018 16:11