Sequences from confirmation amplicon


Confirmation sequences for line 114F11 and hit At5g12200 are shown.
Line ID 114F11
Locus At5g12200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3942795
Primers used for PCR RZ02 - 8474, show primer details
   
Sequence Name 25-K016845-022-114-F11C-8409
GenBank Accession LN497222 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K016845-022-114-F11C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAAATATTGAGAAAAAC
TTTGAAAATCTGAAAGAATAAATGGGAATCATTTGATTAACAGAACTGAAGGATTTATTA
CTTGGACGCAATTGTGAAGTCAGGATCCCAAAGCCAATGATCATCAAGGATTAATCCAGA
CACAACAGGCTCTCCAATAACCTTCTGTCCTGAAATACATAACAAAAAAACTTTTCATAT
TTATTAAGCCAAATACTAAGTAAAAATTTGAAAGGTACTTTTCTACACATATGTTATTGC
ACTAGTGGATGTTCGGATAGGAGATTGATTTCTAACAGGATAATGTCCATACAATGAGAA
AAGCAAAAGGTTAGTCCTACATGAGTTGTAACAGAAAACCTGATTTTCGAGCTTTAGCAA
TCTCGTCCATTGCATCAACACTCATCACATGAACAACATAGAGAGGCGTGTTAATAAAAC
GAGCCAAACGAATTGCTCTAGCAGTGGCCTCTCCCTCGAGCTGACATTGTCAAACAAGGA
TCTTGTTTTAGTAGCTTCAACAAAGCTTTAC
   
Sequence Name 26-K016845-022-114-F11C-RZ02
GenBank Accession LN497697 [GenBank]
Sequencing primer gene specific primer, RZ02: 5'-CTCTTGCCTCTGTAAAGCTTTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K016845-022-114-F11C-rz02
ACTACTAAAACAAGATCCTTGTTTGACAATGTCAGCTCGAGGGAGAGGCCACTGCTAGAG
CAATTCGTTTGGCTCGTTTTATTAACACGCCTCTCTATGTTGTTCATGTGATGAGTGTTG
ATGCAATGGACGAGATTGCTAAAGCTCGAAAATCAGGTTTTCTGTTACAACTCATGTAGG
ACTAACCTTTTGCTTTTCTCATTGTATGGACATTATCCTGTTAGAAATCAATCTCCTATC
CGAACATCCACTAGTGCAATAACATATGTGTAGAAAAGTACCTTTCAAATTTTTACTTAG
TATTTGGCTTAATAAATATGAAAAGTTTTTTTGTTATGTATTTCAGGACAGAAGGTTATT
GGAGAGCCTGTTGTGTCTGGATTAATCCTTGATGATCATTGGCTTTGGGATCCTGACTTC
ACAATTGCGTCCAAGTAATAAATCCTTCAGTTCTGTTAATCAAATGATTCCCATTTATTC
TTTCAGATTTTCAAAGTTTTTCTCAATATTTCAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11