Sequences from confirmation amplicon


Confirmation sequences for line 117F01 and hit At3g26932 are shown.
Line ID 117F01
Locus At3g26932 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9930363
Primers used for PCR HM16 - 8474, show primer details
   
Sequence Name 03-K028918-022-117-F01C-8409
GenBank Accession LN487351 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K028918-022-117-F01C-8409
ATTGAAATATCCTGATAACCATTTCTCTAATCTCTCTATTATCAAATCCTTAACATAGGG
TTTATCTAAAGACACAACCTTTAATCATGGGTTTGATCCAGATTTTGTTTCTAATCCTAA
TTTGGTAATGACTTCTTCTCTTGGCTTCCATTTCCCAGCTCCCTTGTGATGAACGGTGAT
GAATTAGGGTTAGGGTTAGGTTTTGGTGGAGGAGCTGAACAAACTGGGATCACTGATCTG
ATGTGAACTGCTGGAGCTAGTTTCTGATGGTTTAACACAAAACTTGGAAAGGGTAAGAGC
TTACTATAACAGTTTCTCTCCACTGATGATGATAATGGTGATGGTGATGATGTTTTGATT
CTCATCTCTGG
   
Sequence Name 04-K028918-022-117-F01C-HM16
GenBank Accession LN487786 [GenBank]
Sequencing primer gene specific primer, HM16: 5'-AGCCAGAGATGAGAATCAAAACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K028918-022-117-F01C-HM16
GTTATAGTAAGCTCTTACCCTTTCCAAGTTTTGTGTTAAACCATCAGAAACTAGCTCCAG
CAGTTCACATCAGATCAGTGATCCCAGTTTGTTCAGCTCCTCCACCAAAACCTAACCCTA
ACCCTAATTCATCACCGTTCATCACAAGGGAGCTGGGAAATGGAAGCCAAGAGAAGAAGT
CATTACCAAATTAGGATTAGAAACAAAATCTGGATCAAACCCATGATTAAAGGTTGTGTC
TTTAGATAAACCCATGTTAAGGATTTGATAATAGAGAGATTAGAGAAATGGTTATCAGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTACATTC

Last Updated on 06.12.2018 16:11