Sequences from confirmation amplicon


Confirmation sequences for line 125G11 and hit At3g58780 are shown.
Line ID 125G11
Locus At3g58780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21740765
Primers used for PCR PH16 - 8474, show primer details
   
Sequence Name 03-K017453-022-125-G11C-PH16
GenBank Accession LN487271 [GenBank]
Sequencing primer gene specific primer, PH16: 5'-TATGCTGAAGTTTTTCAACCCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K017453-022-125-G11C-PH16
TTTTTATACTTTATATTATGTAGATTTGCTTTTAGGGTAATATGTCCAACTAGAAGTCCT
AAAAATCAATAAACACACGGATGACTTTGTCTAACATTGTTATCAGTCATCAAATGTAAA
ATTGTACAAATAATGAAATTAAAGATTTAGTCTCTTTTATTTTTTTTGTTTAGGGTGTAT
ATATATATATATATGTATATTTGTTGCATTGATATATCAATGAGAGGGAGAGAACTCAGA
GAAGTGACGGAAATTAAAATGGTACGAGCCAATTGGAATCTCTGGCATTCTGAGCTTCAT
TTATTTGTTATTAGAAAAAAAAAAAAAAAAAAGCCCTTAAAAAAACCCTTCTGGAGGACT
TTAAATCTTGAAATATCCCCAAAACGGGGTTTATTCCCCCCCCAACCCCTATTTACCATT
TTCAAAACCAAAATTTTCCCAAGTGGGTTTATATCCCCTCCCTTAAAAACATTGTTTGTT
TAAAACCCCTGGGAAATAAAAAAAAAAAAAAAATTTGGGGGTTTTTTCCACCCCGGGGGA
AAAAAAAATTTAAAAATTTTCCCCCCCCCCGGGGCAAAATTTGGCCCAAAC
   
Sequence Name 04-K017453-022-125-G11C-8409
GenBank Accession LN487714 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K017453-022-125-G11C-8409
CGTTTTGGTACAGAACTTGGCTAACCTCAACTATCAATTGGTACCTGGGTATTAGCTTCG
GTGACGGAAGGAGGGTTGACGGCATCGGAACAAGCTTTCTTGTACCTTTCAATTGTACCC
CTCACACTAATAAGTAAGATCGCGGAAAAAACATTAGATGTATACATTGTAGGAAATTCT
TGATTAATTAGAGTTAATTTCGAAATGTTTTTTTTCCTGAAAAGGGAAGGCTTTGGAGTT
TGGACCAATATCTGACCAGTACGAGGAGAAAATTTATAAATTTATTCTAACACATGGCTT
GAATAGATCCACAAGTTTTTTTTTTTTTCTTATTTCACAGGGTATTATAAACAAACAATG
TCTTAAAGTAGGGATATATAAACATCCTAGTAAATATTTTGGTTCCAAAATTGGAAATTA
GGGTTTGAGGAGGAATTAAACCATGTATCGGGAATATTACATGATT

Last Updated on 06.12.2018 16:11