Sequences from confirmation amplicon


Confirmation sequences for line 127A04 and hit At5g45010 are shown.
Line ID 127A04
Locus At5g45010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18167960
Primers used for PCR HP34 - 8474, show primer details
   
Sequence Name 57-K021167-022-127-A04C-8409
GenBank Accession LN509279 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K021167-022-127-A04C-8409
GATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGATGAATACAACTTCTTAG
TTTGTATAAGACAGTGCAGAATCAAAATTTGTATTTATACCAAAACCTAATCAATCACCA
AATCAGTTGCTAATGAAATTTGCTCTTATCTCCATTAATTTTATAACCGATGATGAATCA
ATTTCGAAAATCAAAGAAGCAATTAGTATAAAACAAAAAAATCACCTTCGTTGATTTCAA
ATTCCTCGAACTCATCGTCGTCCTCGAATAGATCCACCTTCACCACTTCGACAGCTGCCT
TGGGTTCTGCCGCCATAGTTACAGATGTTTAAGCAAATCAACCAGATCGAGAAAAAAACC
TAAGAAGAAGATCACCAAACTCGATTAATTCCTCCAAGTTAAACCCAACCAGAGCTCTTT
AAACGAACCTTTTTTTCGTTTTGGGTCTAACTTGAAGAAGGGGAAAATTACCAATTTCCA
ATAACCGACCCGAATCTTATATGATTCATACCGAATCCGACCCGAATCTTATATGATTCA
TACCGAATCCGACCCAATATTAAAAATATCCGATTGAGGTTTAAAAGAAGCTGTTTTAGA
CCGGTCCAATAGTCGCATTCGTCTTGACCAAATCCATGACATACAAACCGGTCCAAAAGT
TGTCACGCTT
   
Sequence Name 61-K021167-022-127-A04C-HP34
GenBank Accession LN510400 [GenBank]
Sequencing primer gene specific primer, HP34: 5'-AATGGTAATGAGGGATGTCATCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K021167-022-127-A04C-HP34
AAGGAATCCCAAAAAGGACTTTGCTTTGTCTTCACTGCTCTGTCACGCCTAGCGAGTTAA
CTCAGACTAACGAGTTGACTCAGCTGGGTTCTGTGGGGAAAGCGTGACAACTTTTGGACC
GGTTTGTATGTCATGGATTTGGTCAAGACGAATGCGACTATTGGACCGGTCTAAAACAGC
TTCTTTTAAACCTCAATCGGATATTTTTAATATTGGGTCGGATTCGGTATGAATCATATA
AGATTCGGGTCGGATTCGGTATGAATCATATAAGATTCGGGTCGGTTATTGGAAATTGGT
AATTTTCCCCTTCTTCAAGTTAGACCCAAAACGAAAAAAAGGTTCGTTTAAAGAGCTCTG
GTTGGGTTTAACTTGGAGGAATTAATCGAGTTTGGTGATCTTCTTCTTAGGTTTTTTTCT
CGATCTGGTTGATTTGCTTAAACATCTGTAACTATGGCGGCAGAACCCAAGGCAGCTGTC
GAAGTGGTGAAGGTGGATCTATTCGAGGACGACGATGAGTTCGAGGAATTTGAAATCAAC
GAAGGTGATTTTTTTGTTTTATACTAATTGCTTCTTTGATTTTCGAAATTGATTCATCAT
CGGTTATAAAATTAATGGAGATAAGAGCAAATTTCATTAGCAACTGATTTGGTGATTGAT
TAGGTTTTGGTATAAATACAAATTTTGATTCTGC

Last Updated on 06.12.2018 16:11