Sequences from confirmation amplicon


Confirmation sequences for line 127D05 and hit At3g14830 are shown.
Line ID 127D05
Locus At3g14830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4984907
Primers used for PCR 06vo - 3144, show primer details
   
Sequence Name 78-K117997-0022-127-D05C-06vo
GenBank Accession LN513742 [GenBank]
Sequencing primer gene specific primer, 06vo: 5'-GGTAGAAGCTTCACATTCGAGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K117997-0022-127-D05C-06vo
ACTTGGACCCTTCTCTTTCACGCGATTCAACTCTTCTTTTACCGTTACACTTATCAAAAC
AGCACTTGCTCTGGTATGGATATGATAGGAAGGTATGCTTACAAAACTTTGCCCACTTCA
ATGAGTTTTATGTCCTTTTAGAATTCTTCTATCCCAAAACTCATGTAACTCTGCTAATTT
TCGTTGCAGAAAGGTATGCATTCACTCTGTCCAGCTATATGGTCAAAGCATCGGAGGTGG
CTTATGATGTCAATGCTCTCTCTCAATCCTTTAGCTTGCGTAAGCCGCTCTTTCCCGTAA
AGCTGCTATTGTGTTCTCACTTCTATGGAACTGATTCCCTGAACTTGTCATCTATGTGCT
TTGGACAGTCATTTATGGATTTGCAATTTCCAAACGGGCAATTGACGTTCCAACTTCCTT
ATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGG
AGATATCACATCAATCCA
   
Sequence Name 38-K117996-0022-127-D05C-35St
GenBank Accession LN503141 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K117996-0022-127-D05C-35St
CCTTCGCAGACCCTTCCTCTATAAAGGAAGTTGGAACGTCAATTGCCCGTTTGGAAATTG
CAAATCCATAAATGACTGTCCAAAGCACATAGATGACAAGTTCAGGGAATCAGTTCCATA
GAAGTGAGAACACAATAGCAGCTTTACGGGAAAGAGCGGCTTACGCAAGCTAAAGGATTG
AGAGAGAGCATTGACATCATAAGCCACCTCCGATGCTTTGACCATATAGCTGGACAGAGT
GAATGCATACCTTTCTGCAACGAAAATTAGCAGAGTTACATGAGTTTTGGGATAGAAGAA
TTCTAAAAGGACATAAAACTCATTGAAGTGGGCAAAGTTTTGTAAGCATACCTTCCTATC
ATATCCATACCAGAGCAAGTGCTGTTTTGATAAGTGTAACGGTAAAAGAAGAGTTGAATC
GCGTAGAAAGAGAAGTGGTCCAAGTTGGAGAAGAAAGAGAGACGAATTTCCATCGCTTGC
AGTAGAACTCGAATGTGAAGCTTCTACC

Last Updated on 06.12.2018 16:11