Sequences from confirmation amplicon


Confirmation sequences for line 129E11 and hit At4g12250 are shown.
Line ID 129E11
Locus At4g12250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7290915
Primers used for PCR MP06 - 8474, show primer details
   
Sequence Name 51-K017630-022-129-E11C-MP06
GenBank Accession LN507348 [GenBank]
Sequencing primer gene specific primer, MP06: 5'-AGATGCATAACATGTGTGAAGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K017630-022-129-E11C-mp06
AACATCAAAGAGCTTTATGAGTATACGGCGTCGTTTATATCGCCTTCGACTACGAAAACA
CCTGATCTTTCTAAAAGGCCTTGTCTTGCTCGTTTAAGCTTTGGATCGTAGTATCGATTG
AAATTGTCGAGACCTAGAACACCATCGCCGCGTCTTCGTAACGCGATCGAGACGTGAGTA
CCGACGAATCCGGAAGCTCCGGTGACTAATACCGTTAAACCGCCGTGAGACCTAGGACGA
GCGGATTTACGGACTTGTTTCTCCCAGTGAGAGCCGCCGTATTTCGCGGCGGAAATGGAG
GAAGAATCGTTGAGATTCCGGCGAGATGGTGACGGAGGAGGAGATAAAACGAGGTAGAAG
AGGAAGAGAGCGAGGAAGAGAGAAGCCCAAAGGGTGAGTTTTGAGAGACGGAGGTAACGG
TGGTGGTGGAGAATCCCGTACGGTGGAACTTTATCGGTTTTGTACTTTCCGGGAGTAGAA
GGAAGATCATCAAGGTGAGACATTTTCACCGGAGGAGGATTTAGAAATCGGAGAAAATGT
CGATTAGGGTTAGGTTTGGATTTTTGGGTCAGGATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTAT
   
Sequence Name 55-K017630-022-129-E11C-8409
GenBank Accession LN508656 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K017630-022-129-E11C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGACCCAAAAATCC
AAACCTAACCCTAATCGACATTTTCTCCGATTTCTAAATCCTCCTCCGGTGAAAATGTCT
CACCTTGATGATCTTCCTTCTACTCCCGGAAAGTACAAAACCGATAAAGTTCCACCGTAC
GGGATTCTCCACCACCACCGTTACCTCCGTCTCTCAAAACTCACCCTTTGGGCTTCTCTC
TTCCTCGCTCTCTTCCTCTTCTACCTCGTTTTATCTCCTCCTCCGTCACCATCTCGCCGG
AATCTCAACGATTCTTCCTCCATTTCCGCCGCGAAATACGGCGGCTCTCACTGGGAGAAA
CAAGTCCGTAAATCCGCTCGTCCTAGGTCTCACGGCGGTTTAACGGTATTAGTCACCGGA
GCTTCCGGATTCGTCGGTACTCACGTCTCGATCGCGTTACGAAGACGCGGCGATGGTGTT
CTAGGTCTCGACAATTTCAATCGATACTACGATCCAAAGCTTAAACGAGCAAGACAAGGC
CTTTTAGAAAGATCAGGTGTTTTCGTAGTCGAAGGCGATATAAACGACGCCGTTTTACTC
AGAAAGCTCTTTGATGTTGTCCTCTTCACAC

Last Updated on 06.12.2018 16:11