Sequences from confirmation amplicon


Confirmation sequences for line 130E07 and hit At1g16540 are shown.
Line ID 130E07
Locus At1g16540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5665385
Primers used for PCR ZZ07 - 8474, show primer details
   
Sequence Name 25-K020244-022-130-E07C-8409
GenBank Accession LN497246 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K020244-022-130-E07C-8409
CATGTAGATTTCCCGGACATGAAGCCATGTAGATTTACATGAGCTATATTCCAGAGTCTG
AGCTATATTGTTCTTCTCTACAATCTTGAAGGAGTAATACAATGGTATACAGGTCCAGTA
ACAGATTTGTTCTCATATGCTTTTGACGCTTCTCCATATGTTTATACTTTTGATGCTTCT
CCATATGTTTATTTTTATGTATAGTAGACGATAATACACACAAGCTTGAACTTATTCAAT
ATCTGGATTAACTTCTTCCCCAACTCCAATCCAACATTGTCTTTTCTCATCAATCTCGTA
TCTCAAAAGCGTTCCAAACAAGATCTTTCCCTGAATCCAATGACAACACCAAGAAAGGTT
ACTTCACAAATAATTGTCAAATACATTTTTTGTTAATGGAAAAGACTGGTTTTAGAATGT
TGAACCTTTACTCTCCTATATGAAGCTAAAGTTGTTAAGGGCTCATTGGATTTCTTCACT
AGTCCAGCTTCATTACTTATGTTTATCATCTGGCACCGGTTACAACCGCCCAATGACTGC
ATTGAAAGGAACCAACTTTTTAAGAAAACAGAGAACCATGAAGTTTATACTTAATGCATA
TGATTATGGTTGAATCTTGTGTGGATCCATGGACTTACTGTGAAATGATTGTCTCCTATC
TTGACAGTTTTCCATTTATCTTCCCCGTATGGTTCACCTCCAGATATAACCAGATTTGGT
CTGAACCTATGTGGATTGAGTTTTTCAT
   
Sequence Name 26-K020244-022-130-E07C-ZZ07
GenBank Accession LN497721 [GenBank]
Sequencing primer gene specific primer, ZZ07: 5'-TTGTTAATCTCCGAGGAGAGTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K020244-022-130-E07C-ZZ07
CTAAACAGAAGATTAGAAGCAAGTACGTTTCCATGTCCTTTTCATCTTACATCTTTTTCC
TTTTCGCTTGATAAAAACTGATTTGGTTGCAGAAGACGAGGATTACAAACGGGCTCATGA
AAAACTCAATCCACATAGGTTCAGACCAAATCTGGTTATATCTGGAGGTGAACCATACGG
GGAAGATAAATGGAAAACTGTCAAGATAGGAGACAATCATTTCACAGTAAGTCCATGGAT
CCACACAAGATTCAACCATAATCATATGCATTAAGTATAAACTTCATGGTTCTCTGTTTT
CTTAAAAAGTTGGTTCCTTTCAATGCAGTCATTGGGCGGTTGTAACCGGTGCCAGATGAT
AAACATAAGTAATGAAGCTGGACTAGTGAAGAAATCCAATGAGCCCTTAACAACTTTAGC
TTCATATAGGAGAGTAAAGGTTCAACATTCTAAAACCAGTCTTTTCCATTAACAAAAAAT
GTATTTGACAATTATTTGTGAAGTAACCTTTCTTGGTGTTGTCATTGGATTCAGGGAAAG
ATCTTGTTTGGAACGCTTTTGAGATACGAGATTGATGAGAAAAGACAATGTTGGATTGGA
GTTGGGGAAGAAGTTAATCCAGATATTGAATAAGTTCAAGCTTGTGTGTATTATCGTCTA
CTATACATAAAAATAAACATATGGAGAAGCATCAAAAGTATAAACATATGGAGAAGCGTC
AAAAGCATATGAGAACAAATCTGTTACTGGACCTGTATACCATTGTA

Last Updated on 06.12.2018 16:11