Sequences from confirmation amplicon


Confirmation sequences for line 132D09 and hit At3g27690 are shown.
Line ID 132D09
Locus At3g27690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10256103
Primers used for PCR 09lu - 8474, show primer details
   
Sequence Name 45-K114086-0022-132-D09C-09lu
GenBank Accession LN505607 [GenBank]
Sequencing primer gene specific primer, 09lu: 5'-ATATAGCTAATATGCTTTGCGCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K114086-0022-132-D09C-09lu
GCCCTCCTTCTGAGAGATTTGAGATCCTGCCTTGAACCACACGGCTTCACCGAATTTGAC
TCCGTTTTTTGAGAGAATTTCAGGGAAGGTGCAGCCTAAAGCTCCCAACATTGCCCATCT
ACTGTGGATCACTTCGAGCTCACGATTCTTTGCGAATGTTTCTGGATCGGCTGAGAGACC
AGCGGTGTCCCAACCGTAGTCTCCAGGGTATTCTCCGGTTAGGTATGATGGTGTGTTTTC
GGAAAATGGTCCTAGGTATTTGGGACGGTCTGGTCCGTACCTGTCACAAATGTTTTGTTG
GGTACTAAATTAGATGGTTGGCATTTGTAATAAATGGAAAATCTCTTTCAAGAATAACAA
AGATTAGGTCATTTAATAGAGAGAGTGTGAGGTTGGAACCAGATGCTCTGAGGAGTAGAC
TTGACGGTACGACGCATGATGATGCGGCCACCGCCATTGGAGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCACGNTTATTA
   
Sequence Name 69-K114085-0022-132-D09C-8409
GenBank Accession LN512252 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K114085-0022-132-D09C-8409
CCGCATCATCATGCGTCGTACCGTCAAGTCTACTCCTCAGAGCATCTGGTTCCAACCTCA
CACTCTCTCTATTAAATGACCTAATCTTTGTTATTCTTGAAAGAGATTTTCCATTTATTA
CAAATGCCAACCATCTAATTTAGTACCCAACAAAACATTTGTGACAGGTACGGACCAGAC
CGTCCCAAATACCTAGGACCATTTTCCGAAAACACACCATCATACCTAACCGGAGAATAC
CCTGGAGACTACGGTTGGGACACCGCTGGTCTCTCAGCCGATCCAGAAACATTCGCAAAG
AATCGTGAGCTCGAAGTGATCCACAGTAGATGGGCAATGTTGGGAGCTTTAGGCTGCACC
TTCCCTGAAATTCTCTCAAAAAACGGAGTCAAATTCGGTGAAGCCGTGTGGTTCAAGGCA
GGATCTCAAATCTTCTCAGAAGGAGGGCTTGACTACCTCGGAAACCCTAACTTGATCCAC
GCGCAAAGCATATNAGCTATA

Last Updated on 06.12.2018 16:11