Sequences from confirmation amplicon


Confirmation sequences for line 132H10 and hit K18B18 are shown.
Line ID 132H10
Locus K18B18 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23870034
Primers used for PCR CR16 - 8474, show primer details
   
Sequence Name 41-K017120-022-132-H10C-CR16
GenBank Accession LN503891 [GenBank]
Sequencing primer gene specific primer, CR16: 5'-ATGAATGCTTCTCAATCTGGCTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K017120-022-132-H10C-CR16
GCATCAACCGAGTTTGCTTATGGAGCAGGTCATGTAGACCCAATAGCTGCTACTAATCCT
GGACTCGTCTATGAGATAACCAAAACAGATTACTTTGCCTTCCTCTGCGGCATGAACTAC
AATAAGACTACCGTGAAACTCATCTCTGGTGAAGCCGTCACTTGCAGTGAAAAAATCTCA
CCTAGAAACCTCAACTATCCATCAATGTCGGCTAAATTATCCGGATCTAATATCTCCTTC
ATCGTCACTTTCAACAGAACCGTCACCAACGTTGGTACCCCAAACTCTACATACAAATCA
AAAGTTGTCTTAAATCACGGATCTAAGCTCAACGTCAAGGTCTCACCTAGTGTTCTATCT
ATGAAGTCGATGAACGAGAAGCAGTCTTTCACTGTGACTGTTTCGGCCAGTGAGCTCCAT
TCAGAACTGCCTTCGTCTGCAAATCTAATTTGGTCTGATGGAACTCATAATGTGAGAAGC
CCCATTGTTGTTTATACTGGTGACTTCTCACAACCATCTTCATCATAAACCATTATTGTT
GATGTTATAAATTATTCAATTTTGGTTATAGTATGAATGTTTGTTGATGTCTAAGGACAT
TGAATAAGATGATTCAACTGTTGTGTTATCTCATTGGACATGTTCCTCTTATTGTATTCA
ACATAACACATGTCACGAACTGAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAA
   
Sequence Name 42-K017120-022-132-H10C-8409
GenBank Accession LN504283 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K017120-022-132-H10C-8409
TAGATTTCCCGGACATGAAGCCATTTTCAGTTCGTGACATGTGTTATGTTGAATACAATA
AGAGGAACATGTCCAATGAGATAACACAACAGTTGAATCATCTTATTCAATGTCCTTAGA
CATCAACAAACATTCATACTATAACCAAAATTGAATAATTTATAACATCAACAATAATGG
TTTATGATGAAGATGGTTGTGAGAAGTCACCAGTATAAACAACAATGGGGCTTCTCACAT
TATGAGTTCCATCAGACCAAATTAGATTTGCAGACGAAGGCAGTTCTGAATGGAGCTCAC
TGGCCGAAACAGTCACAGTGAAAGACTGCTTCTCGTTCATCGACTTCATAGATAGAACAC
TAGGTGAGACCTTGACGTTGAGCTTAGATCCGTGATTTAAGACAACTTTTGATTTGTATG
TAGAGTTTGGGGTACCAACGTTGGTGACGGTTCTGTTGAAAGTGACGATGAAGGAGATAT
TAGATCCGGATAATTTAGCCGACATTGATGGATAGTTGAGGTTTCTAGGTGAGATTTTTT
CACTGCAAGTGACGGCTTCACCAGAGATGAGTTTCACGGTAGTCTTATTGTAGTTCATGC
CGCAGAGGAAGGCAAAGTAATCTGTTTTGGTTATCTCATAGACGAGTCCAGGATTAGTAG
CAGCTATTGGGTCTACATGACCTGCTCCATAAGCAAACTCGGTTGATGCATAGCCAGATT

Last Updated on 06.12.2018 16:11