Sequences from confirmation amplicon


Confirmation sequences for line 134B10 and hit At2g38170 are shown.
Line ID 134B10
Locus At2g38170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15991182
Primers used for PCR RK17 - 8474, show primer details
   
Sequence Name 19-K015890-022-134-B10C-8409
GenBank Accession LN494541 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K015890-022-134-B10C-8409
GATTTCCCGGACATGAAGCCATTTACAAGAACAGGTAACTGAAATCTATACACACAAGAT
AACAGAAGAATGTTATATAGTAGCGATATATATTATTGTATTCTAATGGATCTTTGATAC
GAAATTTGGTACAGGAAGATGAGTATGATGACGATGTGGAGCAAGAAACCGCGGTGATTA
GTTTTTGGAGCGGTTTTGCATGGTTGGTTGGGATGACACTCGTTATCGCATTGCTATCGG
AGTATGTTGTAGCCACGATTGAGGTAACTCTTTTGAATTCCACATTGAAACTGACATGAT
CAACTAAAACATTATAGTAAATACACTTATCGCCAATTGGTTTTGAGTTGGAAACATGCT
CGCTAATATAACGTCAACTTTGTTTAGGAAGCATCGGATAAATGGAACTTATCAGTGAGT
TTCCTAAGCATCATATTGCTTCCTATTGCTGGAAATGCAGCTGAACATGCTGGAGCCGTT
ATTTTTGCCCTTTAAGAACAAGCTTGTAACTTCGATTTTATTCCTTCAAATTATCGCTTT
TAATCGATCCCGAGATCTATTA
   
Sequence Name 20-K015890-022-134-B10C-RK17
GenBank Accession LN494991 [GenBank]
Sequencing primer gene specific primer, RK17: 5'-GATGATTATGGAAACAGCAAGACA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K015890-022-134-B10C-rk17
CCAGTTTCGAGGGGACCAAAATTGAGATCCATATTAATTCCCAAAATCCACGCCACGATG
ATGGTCAAGGGGACAACGAATAAGCCAATCTGAGTCGCAGAGCCTAACGCAACTCCCAAA
GATATGTCCTAGAAATTATTTGGATTGATATAAGTTCTTGCATTGTGTCATTATATAATA
GATCTCGGGATCGATTAAAAGCGATAATTTTAAGGAATAAAATCGAACTTACAAGCTTGT
TCTTAAAGGCAAAAATAACGGCTCCAGCATGTTCAGCTGCATTTCCAACAATAGGAAGCA
ATATGATGCTTATGAAACTCACTGATAAGTTCCATTTATCCGATGCTTCCTAAACAAAGT
TGACGTTATATTAGCGAGCATGTTTCCAACTCAAAACCAATTGGCGATAAGTGTATTTAC
TATAATGTTTTAGTTGATCATGTCAGTTTCAATGTGGAATTCAAAAGAGTTACCTCAATC
GTGGCTACAACATACTCCGATAGCAATGCGATAACGAGTGTCATCCCAACCAACCATGCA
AAACCGCTCCAAAAACTAATCACCGCGGTTTCTTGCTCCACATCGTCATCATACTCATCT
TCCTGTACCAAATTTCGTATCAAAGATCCATTAGAATACAATAATATATATCGCTACTAT
ATAACATTCTTCTGTTATCTTGTGTGTATAGATTTCAGTTACCTGTTCTTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA

Last Updated on 06.12.2018 16:11