Sequences from confirmation amplicon


Confirmation sequences for line 137C10 and hit At2g24600 are shown.
Line ID 137C10
Locus At2g24600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10454306
Primers used for PCR CN15 - 8474, show primer details
   
Sequence Name 44-K116770-0022-137-C10C-CN15
GenBank Accession LN505310 [GenBank]
Sequencing primer gene specific primer, CN15: 5'-AGTAGATCCACTTGATAAGGCGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K116770-0022-137-C10C-CN15
CGATGAATTTGGCAGCTTCCATGAAAATGCTAACGAAAGCTAAGTGGAGAGGTGTTTGGT
TCTTGTTATTGCGTGCGGAATATAGTTCCAATCCAGTGTCTAACATCTGCATAACTATGT
TTACGTCTCCAAGAAGAGCAGCAAGATGCAAAGGTGTGTCTCCGTACGCGTTACGGGAAG
AGAGAAGGGAAGGTCGGAGCTCAATAATCTTGGCGACCAGTTCTCGGTGACCGAGCTTTG
CAGCTACGTGCAAAACCGTGTTGTTGGTATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 20-K116769-0022-137-C10C-8409
GenBank Accession LN495377 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K116769-0022-137-C10C-8409
AACAACACGGGTTTTGCACGTAGCTGCAAAGCTCGGTCACCGAGAACTGGTCGCCAAGAT
TATTGAGCTCCGACCTTCCCTTCTCTCTTCCCGTAACGCGTACGGAGACACACCTTTGCA
TCTTGCTGCTCTTCTTGGAGACGTAAACATAGTTATGCAGATGTTAGACACTGGATTGGA
ACTATATTCCGCACGCAATAACAAGAACCAAACACCTCTCCACTTAGCTTTCGTTAGCAT
TTTCATGGAAGCTGCCAAATTCATCGTTGAAAAGACGAATTCAGTCGACCTTGATGAACT
CAATTTCGCCTTATCAGTGGGATCTACT

Last Updated on 06.12.2018 16:11