Sequences from confirmation amplicon


Confirmation sequences for line 143A01 and hit At1g01230 are shown.
Line ID 143A01
Locus At1g01230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:97529
Primers used for PCR BK87 - 8474, show primer details
   
Sequence Name 19-K104985-0022-143-A01C-BK87
GenBank Accession LN494753 [GenBank]
Sequencing primer gene specific primer, BK87: 5'-ACTGTATCCTAATTCGAAGCCTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K104985-0022-143-A01C-BK87
CTGATCATCAGAAGGTAATTGCCTCTTCATTCGAACCCTAAACCAACTAAAAAATTGCTA
ATAACCAAAGAGATCAATTCAAACACTAGAGCCAGATTCACATCCGGATCTCAGGAAATA
AAAAAAAAACAAGGAATCAATCCATCATCTAATTTAAAGTCACAACCCTAAGTGAAAAAA
TCATCTCTAAAGCATAAACACAATCGAAAACTTACAACGAAGTGAGCGAGATTAACAACA
GTCCAAGCCATTCCAGGGGAACAACCAGAGACAGAGAGAACAACGAGCCAACCGAAAAAA
AGAATAAGCATGTAAGTCGTCCAAACTCCTGGATACATGAACCATTCCGTATTCCTATTC
ATATCCGGGGGTGGAACCGCTTTCACATACAGATTCCCCATTTCTTCTTCAATCAAATCG
GATCGGATCGAATCTCGTCTTCTTTTGATCAAAATCTTATATTAACACCTGAACTAAAAA
CCCAAATTCACAAAATTCTCTATTTCGGCGAGATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAA
   
Sequence Name 03-K104986-0022-143-A01C-8409
GenBank Accession LN487481 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K104986-0022-143-A01C-8409
TGTGAATCTGGGTTTTAGTTCAGGTGTTAATATAAGATTTTGATCAAAAGAAGACGAGAT
TCGATCCGATCCGATCTGATTGAAGAAGAAATGGCGAATCTGTATGTGAAAGCGGTTCCA
CCACCGGATATGAATAGGAATACGGAATGGTTCATGTATCCAGGAGTTTGGACGACTTAC
ATGCTTATTCTCTTCTTCGGTTGGCTCGTTGTTCTCTCTGTCTCTGGTTGTTCCCCTGGA
ATGGCTTGGACTGTTGTTAATCTCGCTCACTTCGTTGTAAGTCTTCGATTGTGTTTATGC
TTTAGAGATGATTTTTTCACTTAGGGTTGTGACTTTAAATTAGATGATGGATTGATTCCT
TGTTTTTTTTTTATTTCCTGAAATCTGGATGTGAATCTGGCTCTAGTGTTTGAATTGATC
TCTTTGGTTATTAGCAATTTTTTAGTTGGTTTAGGGTTCGAATGAAAAGGCAATTACCTT
CTGATTGATCAGTTTTAGCTATCGTATATCGTTGAGCTTGGATCAGGCTTC

Last Updated on 06.12.2018 16:11