Sequences from confirmation amplicon


Confirmation sequences for line 144C03 and hit At1g20600 are shown.
Line ID 144C03
Locus At1g20600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7133619
Primers used for PCR 48ux - 3144, show primer details
   
Sequence Name 69-K117671-0022-144-C03C-48ux
GenBank Accession LN512276 [GenBank]
Sequencing primer gene specific primer, 48ux: 5'-TTATGGATCGTGAAGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K117671-0022-144-C03C-48ux
TTCATTATTACTAGTAAGCCAAGCTAATCAAAAGCAGTCTAGGAAGAGGGAAGAAAAAAC
TGAAAAAAATCAACCTAAGAGGGTTAAGAATCAGAACATCATGAAAATAAATATCCATGA
CTTTTCAGAAGAAACTCTGAGGTTAATTGAGGATTGGTACAACGCTGAACTCGATCCACA
GGATATATTCGGCGATGATGAGGTGACGAGGCGATTCAGCCGACCGATAAAGAAGCAGTT
GATGTCGAGCGACGTCGATACAGATCAAAGCAGGCTCATGTTATCGAAGGAGCAAGTGAA
GGAGAAGATGCTTCCTTTTCTAGAAGAATCAGAGGATCCGGTAAAGGGGGTTGATGTTTC
GGTCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCC
TGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTG
GGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCAC
   
Sequence Name 21-K117670-0022-144-C03C-3144
GenBank Accession LN495838 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K117670-0022-144-C03C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGACCGAAACAT
CAACCCCCTTTACCGGATCCTCTGATTCTTCTAGAAAAGGAAGCATCTTCTCCTTCACTT
GCTCCTTCGATAACATGAGCCTGCTTTGATCTGTATCGACGTCGCTCGACATCAACTGCT
TCTTTATCGGTCGGCTGAATCGCCTCGTCACCTCATCATCGCCGAATATATCCTGTGGAT
CGAGTTCAGCGTTGTACCAATCCTCAATTAACCTCAGAGTTTCTTCTGAAAAGTCATGGA
TATTTATTTTCATGATGTTCTGATTCTTAACCCTCTTAGGTTGATTTTTTTCAGTTTTTT
CTTCCCTCTTCCTAGACTGCTTTTGATTAGCTTGGCTTACTAGTAATAATGGAAGGGACG
CAACCTCATCATCTATATCGTCTTCATCATCTATATCCTCTTCACGATCCATAA

Last Updated on 06.12.2018 16:11