Sequences from confirmation amplicon


Confirmation sequences for line 145D11 and hit At1g12620 are shown.
Line ID 145D11
Locus At1g12620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4295803
Primers used for PCR GE78 - 3144, show primer details
   
Sequence Name 48-K100996-0022-145-D11C-3144
GenBank Accession LN506450 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K100996-0022-145-D11C-3144
TCCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGTTGTGAAAGA
GGGAAAGCTTCGAGAGGCTGAAGAACTGCACAAGGAGATGATACAACGAGGCATATCTCC
TGATACTGTTACGTACACTTCTTTGATAGATGGGTTTTGCAAGGAGAACCAGCTAGATNA
GGCCAACCACATGCTGGATCTGATGGTTAGCAAAGGATGCGGTCCTAATATCAGGACGTT
TAATATCCTCATAAATGGATATTGTAAGGCTAATCTGATTGACGATGGTTTGGAACTCTT
CCGCAAAATGTCTTTGAGAGGAGTGGTTGCAGATACGGTTACTTATAACACTCTNATCCN
AGGATTTTGTGAATTGGGAAAACTTGAAGTTGCCAAAGAACTCTC
   
Sequence Name 48-K101000-0022-145-D11C-GE78
GenBank Accession LN506451 [GenBank]
Sequencing primer gene specific primer, GE78: 5'-ACCATCTCTTGGAAGAGTTCTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K101000-0022-145-D11C-GE78
CATTCAAATCCTTGGATAGAGTGTTATAAGTAACCGTATCTGCAACCACTCCTCTCAAAG
ACATTTTGCGGAAGAGTTCCAAACCATCGTCAATCAGATTAGCCTTACAATATCCATTTA
TGAGGATATTAAACGTCCTGATATTAGGACCGCATCCTTTGCTAACCATCAGATCCAGCA
TGTGGTTGGCCTTATCTAGCTGGTTCTCCTTGCAAAACCCATCTATCAAAGAAGTGTACG
TAACAGTATCAGGAGATATGCCTCGTTGTATCATCTCCTTGTGCAGTTCTTCAGCCTCTC
GAAGCTTTCCCTCTTTCACAACAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAA
TCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGG
GTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCAATNCA
ATCCA
Primers used for PCR FK22 - 8474, show primer details
   
Sequence Name 53-K101270-0022-145-D11C-8409
GenBank Accession LN508091 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K101270-0022-145-D11C-8409
GCATTTACATTGAATATATCCTGGTGTGATTTTCCTTTTGATCATATCCCTCAGCAACTT
GGCACCATCATCCCATCTACCAGCGTAACAAAAGCCTCTAATGAGAGTGGTGTAGATAAT
AATATCAGCTTTGAACCCTTTGATTTCCATTTCATTGAAAAGGTTGAATGCATTGTCGAG
GCTCCCGTCTTTGCACAGACCATCAATGATGATACTGTATTTGACTGCATCGAGCTTGAT
CTTTCTTTCTTCCATCTTTCTGAGCAACTCCATGGCAAGGGCAGTTTGGCCGGACTTACA
CATTACTTTTAAAACCGGTCCATAAGTTACTTCATTGGGTTGAAAGCCAGTCTCAACCAT
TCGATCAATCAAAAGCACAGCATCAGAGACTTTACCGTTGAGACAAAGTCCATTGACCAG
AGCGTTAAGCGTTATGAGAGTGGGTTTATGTCCCATTTCAACCATTCGATCAACTAATTC
CAGAGCTTCGGAAACTCTACCCTCGAGACATAATCCGTTTATCAAAGTTGAAAATGTGAC
TG
   
Sequence Name 53-K101276-0022-145-D11C-FK22
GenBank Accession LN508092 [GenBank]
Sequencing primer gene specific primer, FK22: 5'-TGAGCCTGATACAGTCACATTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K101276-0022-145-D11C-FK22
GTCTCGAGGGAGAGTTTCCGAAGCTCTGGAATTAGTTGATCGAATGGTTGAAATGGGACA
TAAACCCACTCTCATAACGCTTAACGCTCTGGTCAATGGACTTTGTCTCAACGGTAAAGT
CTCTGATGCTGTGCTTTTGATTGATCGAATGGTTGAGACTGGCTTTCAACCCAATGAAGT
AACTTATGGACCGGTTTTAAAAGTAATGTGTAAGTCCGGCCAAACTGCCCTTGCCATGGA
GTTGCTCAGAAAGATGGAAGAAAGAAAGATCAAGCTCGATGCAGTCAAATACAGTATCAT
CATTGATGGTCTGTGCAAAGACGGGAGCCTCGACAATGCATTCAACCTTTTCAATGAAAT
GGAAATCAAAGGGTTCAAAGCTGATATTATTATCTACACCACTCTCATTAGAGGCTTTTG
TTACGCTGGTAGATGGGATGATGGTGCCAAGTTGCTGAGGGATATGATCAAAAGGAAAAT
CACACCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11